The ability to explicate the complicated protein-protein interactions (PPIs) network can greatly advance the understanding of the mechanisms underlying virtually all the cellular events. This review summarizes the chemical and biological strategies developed to monitor, analyze and identify the PPIs in living cells with highlights on their limitations and challenging questions that need to be addressed.

中文翻译：

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分析活细胞中蛋白质-蛋白质相互作用的化学和生物策略

阐明复杂的蛋白质-蛋白质相互作用（PPI）网络的能力可以极大地促进对几乎所有细胞事件背后机制的理解。这篇综述总结了用于监测、分析和识别活细胞中 PPI 的化学和生物策略，并强调了它们的局限性和需要解决的挑战性问题。