The zebrafish telencephalon is composed of highly specialized subregions that regulate complex behaviors such as learning, memory, and social interactions. The transcriptional signatures of the neuronal cell types in the telencephalon and the timeline of their emergence from larva to adult remain largely undescribed. Using an integrated analysis of single-cell transcriptomes of approximately 64,000 cells obtained from 6-day-postfertilization (dpf), 15-dpf, and adult telencephalon, we delineated nine main neuronal cell types in the pallium and eight in the subpallium and nominated novel marker genes. Comparing zebrafish and mouse neuronal cell types revealed both conserved and absent types and marker genes. Mapping of cell types onto a spatial larval reference atlas created a resource for anatomical and functional studies. Using this multiage approach, we discovered that although most neuronal subtypes are established early in the 6-dpf fish, some emerge or expand in number later in development. Analyzing the samples from each age separately revealed further complexity in the data, including several cell types that expand substantially in the adult forebrain and do not form clusters at the larval stages. Together, our work provides a comprehensive transcriptional analysis of the cell types in the zebrafish telencephalon and a resource for dissecting its development and function.

中文翻译：

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成熟斑马鱼端脑的单细胞转录组图谱

斑马鱼端脑由高度专业化的子区域组成，调节学习、记忆和社交互动等复杂行为。端脑中神经元细胞类型的转录特征及其从幼虫到成虫出现的时间线在很大程度上仍未被描述。通过对从受精后 6 天 (dpf)、15-dpf 和成人端脑获得的约 64,000 个细胞的单细胞转录组进行综合分析，我们描绘了大脑皮层中的 9 种主要神经元细胞类型和大脑皮层下的 8 种主要神经元细胞类型，并提名了新的神经元细胞类型。标记基因。比较斑马鱼和小鼠神经元细胞类型揭示了保守和缺失的类型和标记基因。将细胞类型映射到空间幼虫参考图谱上，为解剖学和功能研究创造了资源。使用这种混龄方法，我们发现虽然大多数神经元亚型是在 6-dpf 鱼的早期建立的，但有些神经元亚型在发育后期才出现或数量增加。分别分析每个年龄的样本揭示了数据的进一步复杂性，包括在成体前脑中大量扩增并且在幼虫阶段不形成簇的几种细胞类型。我们的工作共同提供了对斑马鱼端脑细胞类型的全面转录分析，并为剖析其发育和功能提供了资源。