Fig (Ficus carica L.) is one of the oldest fruits that is extensively cultivated worldwide. It has gained a lot of interest among growers due to its unique characteristics, nutritional values, and medicinal properties. Like other fruit crops, each part of the plant is susceptible to fungal diseases that affect fruit production and its quality. Samplings conducted from September 2018 to March 2019 showed that fig plants have been infected by leaf blight, stem canker, and anthracnose fruit rot. The present study aims to determine the causal pathogens of those diseases using morphological, molecular, and pathogenicity analyses. A total of 28 blighted leaves, 11 cankered stems, and 18 anthracnose-rotted fruits of F. carica were collected from different nurseries in several states in Malaysia. The results of morphological characterization, DNA sequences, and phylogenetic analysis of internal transcribed spacer (ITS), β-tubulin (tub2), and glyceraldehyde-3-phosphate dehydrogenase (gapdh) revealed that, of the 39 fungal isolates obtained, they belong to Colletotrichum fructicola (one isolate), C. siamense (30 isolates), and C. truncatum (eight isolates). From the 30 isolates of C. siamense, 10 isolates were obtained from leaves, eight isolates from stems, and 12 isolates from fruits, whereas eight isolates of C. truncatum, and one isolate of C. fructicola were obtained from leaves. The pathogenicity tests conducted on F. carica confirmed that C. fructicola, C. siamense, and C. truncatum were the causal pathogens of leaf blight, while stem canker and anthracnose fruit rot were caused by C. siamense. The present study highlighted three different Colletotrichum species namely C. fructicola, C. siamense, and C. truncatum associated with diseased figs (F. carica) in Malaysia.

无花果 ( Ficus carica L.) 是世界上广泛种植的最古老的水果之一。由于其独特的特性、营养价值和药用价值，它受到了种植者的广泛关注。与其他水果作物一样，植物的每个部分都容易感染影响水果产量及其质量的真菌病害。2018 年 9 月至 2019 年 3 月进行的采样显示，无花果植物受到叶枯病、茎溃疡病和炭疽病果腐病的感染。本研究旨在使用形态学、分子学和致病性分析来确定这些疾病的致病病原体。F. carica共枯萎叶28个，茎干腐烂11个，炭疽病果实18个是从马来西亚几个州的不同托儿所收集的。内部转录间隔区 (ITS)、β-微管蛋白 ( tub2 ) 和甘油醛-3-磷酸脱氢酶 ( gapdh ) 的形态学特征、DNA 序列和系统发育分析结果表明，在获得的 39 株真菌分离株中，它们属于Colletotrichum fructicola（一个分离株）、C. siamense（30 个分离株）和C. truncatum（八个分离株）。从 30 个C. siamense分离株中，10 个分离株来自叶子，8 个分离株来自茎，12 个分离株来自果实，而 8 个C. truncatum分离株，1 个分离株C. fructicola是从叶子中获得的。对F. carica进行的致病性试验证实，C. fructicola、C. siamense和C. truncatum是叶枯病的致病病原体，而茎腐病和炭疽病果腐病是由C. siamense引起的。本研究重点介绍了三种不同的炭疽菌属物种，即与马来西亚患病无花果 ( F. carica ) 相关的C. fructicola、C. siamense和C. truncatum 。