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Ectopic expression of a mechanosensitive channel confers spatiotemporal resolution to ultrasound stimulations of neurons for visual restoration
Nature Nanotechnology ( IF 38.3 ) Pub Date : 2023-04-03 , DOI: 10.1038/s41565-023-01359-6
Sara Cadoni 1 , Charlie Demené 2 , Ignacio Alcala 1 , Matthieu Provansal 1 , Diep Nguyen 1 , Dasha Nelidova 3 , Guillaume Labernède 1 , Jules Lubetzki 1 , Ruben Goulet 1 , Emma Burban 1 , Julie Dégardin 1 , Manuel Simonutti 1 , Gregory Gauvain 1 , Fabrice Arcizet 1 , Olivier Marre 1 , Deniz Dalkara 1 , Botond Roska 3 , José Alain Sahel 1, 4, 5, 6 , Mickael Tanter 2 , Serge Picaud 1
Affiliation  

Remote and precisely controlled activation of the brain is a fundamental challenge in the development of brain–machine interfaces for neurological treatments. Low-frequency ultrasound stimulation can be used to modulate neuronal activity deep in the brain, especially after expressing ultrasound-sensitive proteins. But so far, no study has described an ultrasound-mediated activation strategy whose spatiotemporal resolution and acoustic intensity are compatible with the mandatory needs of brain–machine interfaces, particularly for visual restoration. Here we combined the expression of large-conductance mechanosensitive ion channels with uncustomary high-frequency ultrasonic stimulation to activate retinal or cortical neurons over millisecond durations at a spatiotemporal resolution and acoustic energy deposit compatible with vision restoration. The in vivo sonogenetic activation of the visual cortex generated a behaviour associated with light perception. Our findings demonstrate that sonogenetics can deliver millisecond pattern presentations via an approach less invasive than current brain–machine interfaces for visual restoration.



中文翻译:

机械敏感通道的异位表达赋予神经元超声刺激视觉恢复的时空分辨率

远程和精确控制大脑激活是开发用于神经治疗的脑机接口的基本挑战。低频超声刺激可用于调节大脑深处的神经元活动,尤其是在表达超声敏感蛋白后。但到目前为止,还没有研究描述过一种超声介导的激活策略,其时空分辨率和声学强度与脑机接口的强制性需求兼容,特别是对于视觉恢复。在这里,我们将大电导机械敏感离子通道的表达与非常规的高频超声刺激相结合,以在时空分辨率和与视力恢复兼容的声能沉积下在毫秒持续时间内激活视网膜或皮质神经元。视觉皮层的体内超声激活产生了与光感知相关的行为。我们的研究结果表明,超声遗传学可以通过一种比目前用于视觉恢复的脑机接口侵入性更小的方法提供毫秒模式呈现。

更新日期:2023-04-03
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