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Integration of Metabolomics and Transcriptomics to Reveal the Metabolic Characteristics of Exercise-Improved Bone Mass
Nutrients ( IF 4.546 ) Pub Date : 2023-03-30 , DOI: 10.3390/nu15071694
Jin-Li Hou 1 , Wan-Yu Yang 1 , Qiong Zhang 1 , Hao Feng 1 , Xiao-Bao Wang 1 , Hui Li 1 , Sheng Zhou 2 , Su-Mei Xiao 1, 3
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(1) Background: Exercise is effective in promoting and maintaining bone mass. The aim of this study was to detect the exercise-induced metabolic changes in bone tissue of zebrafish. (2) Methods: Thirty-eight zebrafish (Danio rerio, six months old) were analyzed. The exercise group (n = 19) received 8 weeks of counter-current swimming training. The control group (n = 19) was not subjected to exercise. Mineralization was quantified, and alkaline phosphatase (Alp) and anti-tartrate acid phosphatase (Trap) activities were estimated (n = 12). The metabolomics (n = 12) and transcriptomics (n = 14) data of bone tissue were used for the integration analyses. (3) Results: The results showed that the exercise training improved the bone mineralization of zebrafish, e.g., the exercise group (5.74 × 104 ± 7.63 × 103) had a higher mean optical density than the control group (5.26 × 104 ± 8.56 × 103, p = 0.046) for the caudal vertebrae. The amount of mineralized matrix in scales of the exercised zebrafish was also higher (0.156 ± 0.012 vs. 0.102 ± 0.003, p = 0.005). Both histological staining and biochemical analysis revealed increased Alp activity (0.81 ± 0.26 vs. 0.76 ± 0.01, p = 0.002) and decreased Trap activity (1.34 ± 0.01 vs. 1.36 ± 0.01, p = 0.005) in the exercise group. A total of 103 different metabolites (DMs, VIP ≥ 1, fold change (FC) ≥ 1.20 or ≤0.83, p < 0.050) were identified. Alanine, aspartate and glutamate metabolism, β-alanine metabolism, pyrimidine metabolism, and pantothenate and CoA biosynthesis were the significantly enriched metabolic pathways (p < 0.050). A total of 35 genes (q ≤ 0.050 (BH), |Log2FC| ≥ 0.5) were coenriched with the 103 DMs in the four identified pathways. Protein–protein interaction network analysis of the 35 genes showed that entpd3, entpd1, and cmpk2 were the core genes. (4) Conclusions: The results of this study suggest that alanine, aspartate and glutamate metabolism, β-alanine metabolism, pyrimidine metabolism, and pantothenate and CoA biosynthesis contributed to exercise-induced improvements in bone mass.

中文翻译:

整合代谢组学和转录组学揭示运动改善骨量的代谢特征

(1) 背景:运动对促进和维持骨量有效。本研究的目的是检测运动引起的斑马鱼骨组织代谢变化。(2) 方法:对 38 条斑马鱼(Danio rerio,6 个月大)进行了分析。运动组 (n = 19) 接受了 8 周的逆流游泳训练。对照组 (n = 19) 不进行运动。量化矿化,并估计碱性磷酸酶 (Alp) 和抗酒石酸酸性磷酸酶 (Trap) 活性 (n = 12)。骨组织的代谢组学 (n = 12) 和转录组学 (n = 14) 数据用于整合分析。(3) 结果:结果表明,运动训练提高了斑马鱼的骨矿化,如运动组(5.74×104±7. 63 × 103) 的尾椎平均光密度高于对照组 (5.26 × 104 ± 8.56 × 103, p = 0.046)。运动过的斑马鱼鳞片中矿化基质的数量也更高(0.156 ± 0.012 对 0.102 ± 0.003,p = 0.005)。组织学染色和生化分析均显示运动组的 Alp 活性增加(0.81 ± 0.26 与 0.76 ± 0.01,p = 0.002),Trap 活性降低(1.34 ± 0.01 与 1.36 ± 0.01,p = 0.005)。共鉴定出 103 种不同的代谢物(DM、VIP ≥ 1、倍数变化 (FC) ≥ 1.20 或 ≤ 0.83、p < 0.050)。丙氨酸、天冬氨酸和谷氨酸代谢、β-丙氨酸代谢、嘧啶代谢以及泛酸和 CoA 生物合成是显着丰富的代谢途径 (p < 0.050)。共有 35 个基因 (q ≤ 0.050 (BH),|Log2FC| ≥ 0。5) 在四个确定的途径中与 103 个 DM 共同富集。对 35 个基因的蛋白质-蛋白质相互作用网络分析表明,entpd3、entpd1 和 cmpk2 是核心基因。(4) 结论:本研究结果表明,丙氨酸、天冬氨酸和谷氨酸代谢、β-丙氨酸代谢、嘧啶代谢以及泛酸和 CoA 生物合成有助于运动诱导的骨量改善。
更新日期:2023-03-31
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