Bioaccumulation of PFOA and PFOS in aquatic organisms is increasingly a global environmental problem. The development of biota matrix certified reference materials (CRMs) for PFOA and PFOS is urgently needed to cope with food safety supervision by assuring accurate analysis results. This paper addresses this urgent need by developing a new oyster matrix CRM of PFOA and PFOS; the certified values and expanded uncertainties are 5.63 ± 0.35 and 3.08 ± 0.20 µg/kg. The CRM candidates were prepared by adding PFOA and PFOS into raw oyster meats, followed by freeze-drying, grinding, blending, homogenizing, bottling, and gamma-irradiation. The sample pretreatment strategy of extraction and clean-up was optimized to minimize matrix effects and interferences. The isotope dilution high-performance liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) strategies were employed for certification, homogeneity, and stability assessment. In addition, uncertainties were evaluated by combining the contributions from characterization, homogeneity, and stability.

PFOA 和 PFOS 在水生生物中的生物积累日益成为一个全球性环境问题。迫切需要开发 PFOA 和 PFOS 的生物群基质认证参考物质 (CRM)，以通过确保准确的分析结果来应对食品安全监管。本文通过开发一种新的 PFOA 和 PFOS 牡蛎基质 CRM 解决了这一迫切需求；标准值和扩展不确定度分别为 5.63 ± 0.35 和 3.08 ± 0.20 µg/kg。CRM 候选物的制备方法是将 PFOA 和 PFOS 添加到生牡蛎肉中，然后进行冷冻干燥、研磨、混合、均质化、装瓶和伽马辐照。优化了提取和净化的样品预处理策略，以最大限度地减少基质效应和干扰。采用同位素稀释高效液相色谱-串联质谱 (ID-LC-MS/MS) 策略进行认证、均质性和稳定性评估。此外，通过结合表征、均匀性和稳定性的贡献来评估不确定性。