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Molecular engineering of fluorescence probe for real-time non-destructive visual screening of meat freshness
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2023-03-20 , DOI: 10.1016/j.aca.2023.341125
Huan Ye 1 , Yingjun Ke 1 , Wenlu Li 2 , Beitong Zhu 1 , Lirong Jiang 1 , Xichao Hu 2 , Lintao Zeng 3
Affiliation  

Spoiled meat poses a great challenge to food security and human health, which should be addressed by the early monitoring and warning of the meat freshness. We herein exploited a molecular engineering strategy to construct a set of fluorescence probes (PTPY, PTAC, and PTCN) with phenothiazine as fluorophore and cyanovinyl as recognition site for the facile and efficient monitoring of meat freshness. These probes produce an obvious fluorescence color transition from dark red to bright cyan in response to cadaverine (Cad) through the nucleophilic addition/elimination reaction. The sensing performances were elaborately improved to achieve quick response (16 s), low detection limit (LOD = 3.9 nM), and high contrast fluorescence color change by enhancing the electron-withdrawing strength of cyanovinyl moiety. Furthermore, PTCN test strips were fabricated for portable and naked-eye detection of Cad vapor with fluorescence color change from crimson to cyan, and accurate determination of Cad vapor level with RGB color (red, green, blue) mode analysis. The test strips were employed to detect the freshness of real beef samples, and demonstrated a good capability of non-destructive, non-contact and visual screening meat freshness on site.



中文翻译:

用于肉类新鲜度实时无损可视化筛选的荧光探针分子工程

变质肉类对食品安全和人类健康构成巨大挑战,应通过肉类新鲜度的早期监测和预警来应对。我们在此利用分子工程策略构建了一组荧光探针(PTPYPTACPTCN) 以吩噻嗪为荧光团,氰基乙烯基为识别位​​点,可轻松有效地监测肉类新鲜度。这些探针通过亲核加成/消除反应响应于尸胺 (Cad),产生从深红色到亮青色的明显荧光颜色转变。通过增强氰基乙烯基部分的吸电子强度,精心改进了传感性能,以实现快速响应(16 秒)、低检测限(LOD = 3.9 nM)和高对比度荧光颜色变化。此外,PTCN制备了荧光颜色由深红色变为青色的Cad蒸气便携式肉眼检测试纸,并通过RGB颜色(红、绿、蓝)模式分析准确测定Cad蒸气含量。该试纸条用于检测真牛肉样品的新鲜度,表现出良好的现场无损、非接触、目视筛查肉类新鲜度的能力。

更新日期:2023-03-24
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