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A cloth-based single-working-electrode electrochemiluminescence sensor for simultaneous detection of diabetes complication markers
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2023-03-20 , DOI: 10.1016/j.aca.2023.341121
Wei Lai 1 , Yi Liang 1 , Xinyuan Mao 1 , Kaifa Xue 1 , Chunsun Zhang 1
Affiliation  

As one of the most common noninfectious diseases, diabetes and diabetic complications (DDC) have attracted great attention in the field of life and health. However, simultaneous detection of DDC markers usually requires labor- and time-consuming steps. Here, a novel cloth-based single-working-electrode electrochemiluminescence (SWE-ECL) sensor was designed for the simultaneous detection of multiple DDC markers. For this sensor, three independent ECL cells are distributed on the SWE, which is a simplification of the configuration of traditional sensors for simultaneous detection. In this way, the modification processes and ECL reactions occur at the back of the SWE, eliminating the adverse effects caused by human intervention on the electrode. Under optimized conditions, glucose, uric acid and lactate were determined, with corresponding linear dynamic ranges of 80–4000 μM, 45–1200 μM and 60–2000 μM, and detection limits of 54.79 μM, 23.95 μM and 25.82 μM, respectively. In addition, the cloth-based SWE-ECL sensor exhibited good specificity and satisfactory reproducibility, and its actual application potential was verified by measuring complex human serum samples. Overall, this work developed a simple, sensitive, low-cost and rapid method for the simultaneous quantitative determination of multiple markers related to DDC and demonstrated a new route for multiple-marker detection.



中文翻译:

用于同时检测糖尿病并发症标志物的布基单工作电极电化学发光传感器

糖尿病及糖尿病并发症(diabetic complications,DDC)作为最常见的非感染性疾病之一,在生命健康领域备受关注。然而,同时检测 DDC 标记通常需要费时费力的步骤。在这里,一种新型的基于布的单工作电极电化学发光 (SWE-ECL) 传感器被设计用于同时检测多个 DDC 标记。对于该传感器,三个独立的 ECL 单元分布在 SWE 上,这是对用于同时检测的传统传感器配置的简化。这样,修饰过程和ECL反应发生在SWE的后面,消除了人为干预对电极造成的不利影响。在优化条件下测定葡萄糖、尿酸和乳酸,相应的线性动态范围为 80-4000 μM、45-1200 μM 和 60-2000 μM,检测限分别为 54.79 μM、23.95 μM 和 25.82 μM。此外,布基SWE-ECL传感器表现出良好的特异性和令人满意的重现性,其实际应用潜力已通过测量复杂的人血清样品得到验证。总的来说,这项工作开发了一种简单、灵敏、低成本和快速的方法来同时定量测定与 DDC 相关的多个标记,并展示了多标记检测的新途径。并通过测量复杂的人血清样本验证了其实际应用潜力。总的来说,这项工作开发了一种简单、灵敏、低成本和快速的方法来同时定量测定与 DDC 相关的多个标记,并展示了多标记检测的新途径。并通过测量复杂的人血清样本验证了其实际应用潜力。总的来说,这项工作开发了一种简单、灵敏、低成本和快速的方法来同时定量测定与 DDC 相关的多个标记,并展示了多标记检测的新途径。

更新日期:2023-03-20
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