The element labeling inductively coupled plasma mass spectrometry (ICP-MS) strategy has been increasingly applied to the bioanalysis for various bio-targets. Herein, a renewable analysis platform with element labeling ICP-MS was firstly proposed for microRNA (miRNA) analysis. The analysis platform was established on the magnetic bead (MB) with entropy-driven catalytic (EDC) amplification. When the EDC reaction was initiated by target miRNA, numerous strands labeled with Ho element were released from MBs, and ¹⁶⁵Ho in the supernatant detected by ICP-MS could reflect the amount of target miRNA. After detection, the platform was easily regenerated by adding strands to reassemble EDC complex on MBs. This MB platform could be used four times, and the limit of detection for miRNA-155 was 8.4 pmol L⁻¹. Moreover, the developed regeneration strategy based on EDC reaction can be easily expanded to other renewable analysis platforms, such as, the renewable platform involving EDC and rolling circle amplification technology. Overall, this work proposed a novel regenerated bioanalysis strategy to reduce the consumption of reagent and time for probe preparation, profiting the development of bioassay based on element labeling ICP-MS strategy.

元素标记电感耦合等离子体质谱 (ICP-MS) 策略已越来越多地应用于各种生物靶标的生物分析。在此，首次提出了用于 microRNA (miRNA) 分析的具有元素标记 ICP-MS 的可再生分析平台。分析平台建立在具有熵驱动催化（EDC）放大功能的磁珠（MB）上。当目标miRNA启动EDC反应时，MBs释放出大量Ho元素标记的链， ICP-MS检测上清液中的¹⁶⁵ Ho可以反映目标miRNA的数量。检测后，通过添加链以在 MB 上重新组装 EDC 复合物，可以轻松地再生该平台。该MB平台可重复使用4次，miRNA-155的检测限为8.4 pmol L ⁻¹. 此外，基于EDC反应开发的再生策略可以很容易地扩展到其他可再生分析平台，例如涉及EDC和滚环扩增技术的可再生平台。总的来说，这项工作提出了一种新的再生生物分析策略，以减少试剂消耗和探针制备时间，有利于基于元素标记 ICP-MS 策略的生物测定的发展。