Chlorpyrifos (CPF) as a classic organophosphorus pesticide has been widely used in agricultural applications to control insects and worms. CPF in the environment can cause deaths of diverse kinds of aquatic organism and bring a high risk to human health. Therefore, the development of effective analytical method for CPF is of great importance. In this work, a novel dual-mode albumin (ALB)-based supramolecular probe FD@ALB was designed and prepared for rapid detection of CPF in the environment. The limit of detection is 0.57 μM (∼ 0.2 ppm) with a wider detection range up to 200 μM, which is satisfactory for application. The sensing mechanism can be ascribed to CPF-induced phosphorylation of ALB, thus leading to a change in the binding microenvironment of FD dye. Moreover, the paper-based test strips were used in conjunction with the FD@ALB, realizing the portable detection of CPF. This method was demonstrated to be suitable for on-site detection of CPF in various kinds of environmental samples, including water, soil, and food samples, with the aid of a smartphone. To the best of our knowledge, this is the first analytical method achieving a combination of the rapid and ratiometric detection of CPF in the environment.

毒死蜱（CPF）作为一种经典的有机磷农药，已广泛用于农业应用中以控制昆虫和蠕虫。环境中的CPF可导致多种水生生物死亡，给人类健康带来较高风险。因此，开发有效的CPF分析方法具有重要意义。本工作设计并制备了一种新型双模式白蛋白（ALB）超分子探针FD@ALB，用于环境中CPF的快速检测。检测限为0.57 μM (∼ 0.2 ppm)，检测范围更宽达200 μM，满足应用需求。传感机制可归因于 CPF 诱导的 ALB 磷酸化，从而导致 FD 染料的结合微环境发生变化。此外，纸质试纸条与FD@ALB配合使用，实现了CPF的便携式检测。该方法被证明适用于借助智能手机对各种环境样品（包括水、土壤和食品样品）中的 CPF 进行现场检测。据我们所知，这是第一个实现环境中 CPF 快速检测和比例检测相结合的分析方法。