The incidence of lung cancer is increasing year by year. The study of the proliferation and metastasis of lung adenocarcinoma cells is of positive significance to improve the prognosis of patients with lung adenocarcinoma, but there is still a lack of more effective treatment for the proliferation and metastasis of lung adenocarcinoma cells. The present study found that a lymphocyte lineage specific transcription factor early B‑cell factor 1 (EBF1), was frequently expressed in human lung cancer tissues. EBF1 short hairpin RNA and knocked down EBF1 expression in lung adenocarcinoma cell line A549 could inhibit the proliferation of lung adenocarcinoma cells in vitro and inhibit the growth of tumors in vivo. The effects of EBF1 expression on the proliferation of lung cancer cells were examined by cell proliferation assay, cell cycle assay and in vivo animal experiments in mice, to explore the possible molecular mechanism of EBF1 involvement in lung cancer cell proliferation. Its mechanism may be related to its influence by blocking cell cycle in G₁ phase, which involves the decrease of cyclin dependent kinase 6 expression and the upregulation of P21/P27 expression. The present study will supplement the hypothesis that the heterotopic expression of hematogenous transcription factors in lung cancer affects tumor proliferation and discover new molecular targets for cancer therapy.

中文翻译：

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shRNA 介导的敲低 EBF1 基因表达对体外和体内肺癌细胞系 A549 增殖的影响。

肺癌的发病率逐年上升。研究肺腺癌细胞的增殖和转移对改善肺腺癌患者的预后具有积极意义，但目前仍缺乏更有效的治疗肺腺癌细胞增殖和转移的方法。本研究发现，淋巴细胞谱系特异性转录因子早期 B 细胞因子 1 ( EBF1 ) 在人类肺癌组织中频繁表达。EBF1短发夹RNA及敲低肺腺癌细胞系A549中EBF1表达可抑制体外肺腺癌细胞增殖，体内抑制肿瘤生长. 通过细胞增殖试验、细胞周期试验和小鼠体内动物实验检测EBF1表达对肺癌细胞增殖的影响，探讨EBF1参与肺癌细胞增殖的可能分子机制。_{其作用机制可能与其通过阻断细胞周期G 1}期影响有关，包括细胞周期蛋白依赖性激酶6表达减少和P21/P27表达上调。本研究将补充肺癌中血源性转录因子的异位表达影响肿瘤增殖的假设，并发现新的癌症治疗分子靶点。