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Transcriptomic analysis reveals mechanisms for the different drought tolerance of sweet potatoes
Frontiers in Plant Science ( IF 5.6 ) Pub Date : 2023-03-16 , DOI: 10.3389/fpls.2023.1136709
Enliang Liu 1 , Linli Xu 2 , Zhengqian Luo 2 , Zhiqiang Li 1 , Guohui Zhou 3 , Haifeng Gao 4 , Furong Fang 1 , Jun Tang 5 , Yue Zhao 1 , Zhilin Zhou 5 , Ping Jin 2
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Drought is a common environmental stress with great negative impacts on plant growth, development and geographical distribution as well as agriculture and food production. Sweet potato is characterized by starchy, fresh and pigmented tuber, and is regarded as the seventh most important food crop. However, there has been no comprehensive study of the drought tolerance mechanism of different sweet potato cultivars to date. Here, we studied the mechanism for drought response of seven sweet potato drought-tolerant cultivars using the drought coefficients, physiological indicators and transcriptome sequencing. The seven sweet potato cultivars were classified into four groups of drought tolerance performance. A large number of new genes and transcripts were identified, with an average of about 8000 new genes per sample. Alternative splicing events in sweet potato, which were dominated by first exon and last exon alternative splicing, were not conserved among different cultivars and not significantly affected by drought stress. Furthermore, different drought-tolerance mechanisms were revealed through differentially expressed gene analysis and functional annotation. Two drought-sensitive cultivars, Shangshu-9 and Xushu-22, mainly resisted drought stress by up-regulating plant signal transduction. The other drought-sensitive cultivar Jishu-26 responded to drought stress by down-regulating isoquinoline alkaloid biosynthesis and nitrogen/carbohydrate metabolism. In addition, the drought-tolerant cultivar Chaoshu-1 and drought-preferred cultivar Z15-1 only shared 9% of differentially expressed genes, as well as many opposite metabolic pathways in response to drought. They mainly regulated flavonoid and carbohydrate biosynthesis/metabolism in response to drought, while Z15-1 increased photosynthesis and carbon fixation capacity. The other drought-tolerant cultivar Xushu-18 responded to drought stress by regulating the isoquinoline alkaloid biosynthesis and nitrogen/carbohydrate metabolism. The extremely drought-tolerant cultivar Xuzi-8 was almost unaffected by drought stress and responded to drought environment only by regulating the cell wall. These findings provide important information for the selection of sweet potatoes for specific purposes.

中文翻译:

转录组学分析揭示了甘薯不同耐旱性的机制

干旱是一种常见的环境胁迫,对植物生长发育和地理分布以及农业和粮食生产都有很大的负面影响。甘薯的特点是块茎富含淀粉、新鲜且有色素,被认为是第七大最重要的粮食作物。然而,迄今为止还没有对不同甘薯品种的耐旱机制进行全面的研究。在这里,我们利用干旱系数、生理指标和转录组测序研究了七个甘薯耐旱品种的干旱响应机制。七个甘薯品种被分为四组耐旱性能。鉴定出大量新基因和转录本,平均每个样本约有 8000 个新基因。以第一外显子和最后外显子可变剪接为主的甘薯可变剪接事件在不同品种间不保守,受干旱胁迫影响不显着。此外,通过差异表达基因分析和功能注释揭示了不同的耐旱机制。Shangshu-9 和 Xushu-22 两个干旱敏感品种主要通过上调植物信号转导来抵抗干旱胁迫。另一个干旱敏感品种 Jishu-26 通过下调异喹啉生物碱生物合成和氮/碳水化合物代谢来应对干旱胁迫。此外,耐旱品种朝蜀1号和喜旱品种Z15-1仅有9%的差异表达基因,以及许多相反的干旱代谢途径。它们主要调节类黄酮和碳水化合物的生物合成/代谢以应对干旱,而 Z15-1 增加光合作用和固碳能力。另一个耐旱品种徐蜀 18 通过调节异喹啉生物碱的生物合成和氮/碳水化合物代谢来应对干旱胁迫。极其耐旱的品种旭子8几乎不受干旱胁迫的影响,仅通过调节细胞壁来响应干旱环境。这些发现为特定用途的红薯选择提供了重要信息。另一个耐旱品种徐蜀 18 通过调节异喹啉生物碱的生物合成和氮/碳水化合物代谢来应对干旱胁迫。极其耐旱的品种旭子8几乎不受干旱胁迫的影响,仅通过调节细胞壁来响应干旱环境。这些发现为特定用途的红薯选择提供了重要信息。另一个耐旱品种徐蜀 18 通过调节异喹啉生物碱的生物合成和氮/碳水化合物代谢来应对干旱胁迫。极其耐旱的品种旭子8几乎不受干旱胁迫的影响,仅通过调节细胞壁来响应干旱环境。这些发现为特定用途的红薯选择提供了重要信息。
更新日期:2023-03-16
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