Nicotiana benthamiana is increasingly used for transient gene expression to produce antibodies, vaccines, and other pharmaceutical proteins but transient gene expression is low in fully developed, 6–8-week old plants. This low gene expression is thought to be caused by the perception of the cold shock protein (CSP) of Agrobacterium tumefaciens. The CSP receptor is contested because both NbCSPR and NbCORE have been claimed to perceive CSP. Here, we demonstrate that CSP perception is abolished in 6-week-old plants silenced for NbCORE but not NbCSPR. Importantly, older NbCORE-silenced plants support a highly increased level of GFP fluorescence and protein upon agroinfiltration. The drastic increase in transient protein production in NbCORE-depleted plants offers new opportunities for molecular farming, where older plants with larger biomass can now be used for efficient protein expression.

中文翻译：

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NbCORE 受体的耗尽显着提高了老烟草植物的农杆菌渗透生产力

本塞姆氏烟草越来越多地用于瞬时基因表达以生产抗体、疫苗和其他药物蛋白，但瞬时基因表达在完全发育的 6-8 周龄植物中较低。这种低基因表达被认为是由根癌农杆菌的冷休克蛋白 (CSP) 的感知引起的。CSP 受体是有争议的，因为据称Nb CSPR 和Nb CORE 都可以感知 CSP。在这里，我们证明 CSP 感知在 6 周大的植物中被废除，这些植物对Nb CORE 而不是Nb CSPR 沉默。重要的是，较旧的NbCORE 沉默的植物在农杆菌渗入后支持高度增加的 GFP 荧光和蛋白质水平。Nb CORE 耗尽植物中瞬时蛋白质产量的急剧增加为分子农业提供了新的机会，其中生物量更大的老植物现在可以用于有效的蛋白质表达。