A microdots array-based fluoremetric method with superwettability profile has been developed for the simultaneous and separate detection of Fe³⁺ and Cu²⁺ ions in red wine samples. A wettable micropores array was initially designed with high density by using polyacrylic acid (PAA) and hexadecyltrimethoxysilane (HDS), followed by the NaOH etching route. Zinc metal organic frameworks (Zn-MOFs) were fabricated as the fluorescent probes to be immobilized into the micropores array to obtain the fluoremetric microdots array platform. It was found that the fluorescence of Zn-MOFs probes could decrease significantly in the presence of Fe³⁺ and/or Cu²⁺ ions towards their simultaneous analysis. Yet, the specific responses to Fe³⁺ ions could be expected if using histidine to chelate Cu²⁺ ions. Moreover, the developed Zn-MOFs-based microdots array with superwettability profile can enable the accumulation of targeting ions from the complicated samples without any tedious pre-processing. Also, the cross-contamination of different samples droplets can be largely avoided so as to facilitate the analysis of multiple samples. Subsequently, the feasibility of simultaneous and separate detection of Fe³⁺ and Cu²⁺ ions in red wine samples was demonstrated. Such a design of microdots array-based detection platform may promise the wide applications in analyzing Fe³⁺ and/or Cu²⁺ ions in the fields of food safety, environmental monitoring, and medical diseases diagnostics.

开发了一种具有超润湿性的基于微点阵列的荧光测定方法，用于同时和分别检测红酒样品中的Fe ³⁺和 Cu ^2+离子。最初使用聚丙烯酸 (PAA) 和十六烷基三甲氧基硅烷 (HDS) 设计高密度可湿性微孔阵列，然后采用 NaOH 蚀刻路线。锌金属有机框架 (Zn-MOFs) 被制造为荧光探针, 被固定到微孔阵列中, 以获得荧光微点阵列平台。结果发现，在 Fe ³⁺和/或 Cu ²⁺离子存在的情况下，Zn-MOFs 探针的荧光对于它们的同时分析会显着降低。^{然而，对 Fe 3+}的具体反应^{如果使用组氨酸螯合 Cu 2+}离子，则可以预期离子。此外，所开发的具有超润湿性的基于 Zn-MOFs 的微点阵列可以从复杂的样品中积累靶向离子，而无需任何繁琐的预处理。同时，也可以很大程度上避免不同样品液滴的交叉污染，方便多样品分析。随后，论证了同时和分别检测红酒样品中Fe ³⁺和Cu ²⁺离子的可行性。^{这种基于微点阵列的检测平台的设计有望在Fe 3+}和/或Cu ²⁺分析中得到广泛应用离子在食品安全、环境监测和医学疾病诊断等领域。