Background

Organophosphorus pesticides are widely used in agriculture owing to their high effectiveness as insecticides. Among these, diazinon is a common environmental contaminant that acts as an acetylcholinesterase (AChE) enzyme inhibitor. As the current methods are too expensive and time-consuming for routine analysis of diazinon, its trace monitoring by rapid and sensitive methods is critical to protect the environment and human health.

Results

A biosensor was introduced for the indirect detection of diazinon using a molybdenum disulfide/zirconium metal-organic framework (MoS₂@MIP-202(Zr)) nanocomposite. The probe is based on the peroxidase mimic of the prepared nanocomposite on NaHCO₃–H₂O₂ chemiluminescence system as well as the inhibitory effect of diazinon on the enzymatic activity of AChE. The chemiluminescence signal is gradually decreased with an increase in diazinon concentration, and there is a linear relationship between the analytical signal and diazinon concentration. Under the optimum conditions, the calibration plot is linear in the concentration range of 0.5–300.0 nmol L⁻¹. The limit of detection and quantification limit of the method are 0.12 and 0.40 nmol L⁻¹, respectively. The inter-day and intra-day relative standard deviations (% RSD n = 5, diazinon concentration; 100 nmol L⁻¹) are 3.66 and 1.35%, respectively. The method was used for diazinon detection in real water samples, and the high relative recovery values for the spiked samples along with satisfactory results of a certified reference material analysis confirmed that the method is accurate and free from the matrix effect.

Significance and novelty

A nano-probe based on the peroxidase-like property of MoS₂@MIP-202(Zr) nanocomposite was developed for the first time for indirect detection of residue levels of diazinon in water samples. The high stability of the nanocomposite makes it a good alternative for natural peroxidase enzymes such as horseradish peroxidase with low stability.

中文翻译：

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基于二硫化钼/锆金属-有机骨架纳米复合材料类过氧化物酶特性的化学发光生物传感器用于二嗪磷监测

背景

有机磷杀虫剂由于其作为杀虫剂的高效性而被广泛用于农业。其中，二嗪磷是一种常见的环境污染物，可作为乙酰胆碱酯酶 (AChE) 酶抑制剂。由于目前的方法对于二嗪农的常规分析来说过于昂贵和耗时，因此通过快速灵敏的方法对其痕量进行监测对于保护环境和人类健康至关重要。

结果

引入了一种生物传感器，用于使用二硫化钼/锆金属有机骨架 (MoS ₂ @MIP-202(Zr)) 纳米复合材料间接检测二嗪农。该探针是基于制备的纳米复合物在NaHCO ₃ –H ₂ O ₂化学发光体系上的过氧化物酶模拟物以及二嗪农对乙酰胆碱酯酶活性的抑制作用。化学发光信号随着二嗪农浓度的增加而逐渐减弱，分析信号与二嗪农浓度呈线性关系。在最佳条件下，校准曲线在 0.5–300.0 nmol L ^{−1的浓度范围内呈线性}. 该方法的检测限和定量限分别为0.12和0.40 nmol L ^-1。日间和日内相对标准偏差（% RSD n = 5，二嗪农浓度；100 nmol L ^-1）分别为 3.66 和 1.35%。该方法用于实际水样中的二嗪磷检测，加标样品的高相对回收率值以及有证标准物质分析的令人满意的结果证实该方法准确且不受基质效应影响。

意义和新颖性

_{基于MoS 2} @MIP-202(Zr)纳米复合材料的类过氧化物酶特性的纳米探针首次被开发用于间接检测水样中二嗪农的残留水平。纳米复合材料的高稳定性使其成为天然过氧化物酶的良好替代品，例如稳定性低的辣根过氧化物酶。