Cellular mechanical property analysis reflecting the physiological and pathological states of cells plays a crucial role in assessing the quality of stored blood. However, its complex equipment needs, operation difficulty, and clogging issues hinder automated and rapid biomechanical testing. Here, we propose a promising biosensor assisted by magnetically actuated hydrogel stamping to fulfill it. The flexible magnetic actuator triggers the collective deformation of multiple cells in the light-cured hydrogel, and it allows for on-demand bioforce stimulation with the advantages of portability, cost-effectiveness, and simplicity of operation. The magnetically manipulated cell deformation processes are captured by the integrated miniaturized optical imaging system, and the cellular mechanical property parameters are extracted from the captured images for real-time analysis and intelligent sensing. In this work, 30 clinical blood samples with different storage durations (<14 days and >14 days) were tested. A deviation of 3.3% in the differentiation of blood storage durations by this system compared to physician annotation demonstrated its feasibility. This system should broaden the application of cellular mechanical assays in diverse clinical settings.

中文翻译：

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用于存储血液质量检测的磁驱动水凝胶冲压辅助细胞机械分析仪

反映细胞生理和病理状态的细胞力学特性分析对评估储存血液的质量起着至关重要的作用。然而，其复杂的设备需求、操作难度和堵塞问题阻碍了自动化和快速的生物力学测试。在这里，我们提出了一种有前途的生物传感器，通过磁驱动水凝胶冲压来辅助实现它。柔性磁致动器触发光固化水凝胶中多个细胞的集体变形，并允许按需生物力刺激，具有便携、成本效益和操作简单的优点。磁力操纵的细胞变形过程由集成的微型光学成像系统捕获，从拍摄的图像中提取细胞力学性能参数，进行实时分析和智能感知。在这项工作中，测试了 30 个具有不同存储时间（<14 天和 >14 天）的临床血液样本。与医生注释相比，该系统对血液储存时间的区分有 3.3% 的偏差，证明了其可行性。该系统应该扩大细胞力学测定在不同临床环境中的应用。