Sports Medicine ( IF 9.8 ) Pub Date : 2023-03-01 , DOI: 10.1007/s40279-023-01822-3 Jorn Trommelen 1 , Glenn A A van Lieshout 1, 2 , Pardeep Pabla 3 , Jean Nyakayiru 2 , Floris K Hendriks 1 , Joan M Senden 1 , Joy P B Goessens 1 , Janneau M X van Kranenburg 1 , Annemie P Gijsen 1 , Lex B Verdijk 1 , Lisette C P G M de Groot 4 , Luc J C van Loon 1
Background
Casein protein ingestion prior to sleep has been shown to increase myofibrillar protein synthesis rates during overnight sleep. It remains to be assessed whether pre-sleep protein ingestion can also increase mitochondrial protein synthesis rates. Though it has been suggested that casein protein may be preferred as a pre-sleep protein source, no study has compared the impact of pre-sleep whey versus casein ingestion on overnight muscle protein synthesis rates.
Objective
We aimed to assess the impact of casein and whey protein ingestion prior to sleep on mitochondrial and myofibrillar protein synthesis rates during overnight recovery from a bout of endurance-type exercise.
Methods
Thirty-six healthy young men performed a single bout of endurance-type exercise in the evening (19:45 h). Thirty minutes prior to sleep (23:30 h), participants ingested 45 g of casein protein, 45 g of whey protein, or a non-caloric placebo. Continuous intravenous l-[ring-13C6]-phenylalanine infusions were applied, with blood and muscle tissue samples being collected to assess overnight mitochondrial and myofibrillar protein synthesis rates.
Results
Pooled protein ingestion resulted in greater mitochondrial (0.087 ± 0.020 vs 0.067 ± 0.016%·h−1, p = 0.005) and myofibrillar (0.060 ± 0.014 vs 0.047 ± 0.011%·h−1, p = 0.012) protein synthesis rates when compared with placebo. Casein and whey protein ingestion did not differ in their capacity to stimulate mitochondrial (0.082 ± 0.019 vs 0.092 ± 0.020%·h−1, p = 0.690) and myofibrillar (0.056 ± 0.009 vs 0.064 ± 0.018%·h−1, p = 0.440) protein synthesis rates.
Conclusions
Protein ingestion prior to sleep increases both mitochondrial and myofibrillar protein synthesis rates during overnight recovery from exercise. The overnight muscle protein synthetic response to whey and casein protein does not differ.
Clinical Trial Registration
NTR7251.
中文翻译:
睡前摄入蛋白质可增加耐力运动过夜恢复期间线粒体蛋白质合成率:一项随机对照试验
背景
研究表明,睡前摄入酪蛋白可以增加夜间睡眠期间肌原纤维蛋白的合成率。睡前摄入蛋白质是否也能增加线粒体蛋白质合成率仍有待评估。尽管有人建议酪蛋白可能是首选的睡前蛋白质来源,但没有研究比较睡前乳清与酪蛋白摄入对夜间肌肉蛋白合成率的影响。
客观的
我们的目的是评估睡前摄入酪蛋白和乳清蛋白对耐力型运动过夜恢复期间线粒体和肌原纤维蛋白合成率的影响。
方法
36 名健康的年轻人在晚上(19:45)进行了一次耐力型运动。睡前 30 分钟(23:30),参与者摄入 45 克酪蛋白、45 克乳清蛋白或无热量安慰剂。应用连续静脉内1- [环- 13 C 6 ]-苯丙氨酸输注,收集血液和肌肉组织样本以评估过夜线粒体和肌原纤维蛋白合成率。
结果
相比之下,混合蛋白质摄入导致更高的线粒体(0.087 ± 0.020 vs 0.067 ± 0.016%·h −1,p = 0.005)和肌原纤维(0.060 ± 0.014 vs 0.047 ± 0.011%·h −1,p = 0.012)蛋白质合成率与安慰剂。摄入酪蛋白和乳清蛋白刺激线粒体(0.082 ± 0.019 vs 0.092 ± 0.020%·h −1 , p = 0.690)和肌原纤维(0.056 ± 0.009 vs 0.064 ± 0.018%·h −1 , p = 0.440)蛋白质合成率。
结论
睡前摄入蛋白质会增加夜间运动恢复期间线粒体和肌原纤维蛋白质的合成率。隔夜肌肉蛋白合成对乳清蛋白和酪蛋白的反应没有差异。
临床试验注册
NTR7251。