当前位置: X-MOL 学术Eur. J. Soil Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Chloroform-labile trace elements in soil via fumigation-extraction: Steps towards the soil microbial ionome beyond C:N:P
European Journal of Soil Science ( IF 4.2 ) Pub Date : 2023-02-27 , DOI: 10.1111/ejss.13356
S. A. Schwalb, K. S. Khan, M. Hemkemeyer, S. Heinze, Z. Oskonbaeva, R. G. Joergensen, F. Wichern

Secondary and trace elements may be limiting soil microbial functioning, albeit microbial demand and content remain largely unknown and methods for their in situ detection are limited. Thus, the objective of the present study was to take the first step towards the method development for the assessment of the soil microbial ionome, that is, the elemental composition of soil microbial communities. Chloroform (CHCl3) fumigation extraction was used for the detection of microbial CHCl3-labile secondary and trace element concentrations in soils. The suitability of two extractants (NH4NO3, CaCl2) for the quantification of CHCl3-labile concentrations of phosphorus, sulphur, potassium, sodium, and magnesium, as well as selenium, iron, zinc, manganese, copper, cobalt, nickel, molybdenum, vanadium, boron, silicon, barium, arsenic, and cadmium, were tested in six agricultural soils. Additionally, three soil to extractant ratios (1:5, 1:10, and 1:20) and two extraction durations, 1 or 2 h, were tested in a subset of two soils. Out of the two extractants tested, 0.01 M CaCl2 was found to be the best-suited extractant. For CaCl2, a soil-to-extractant ratio of 1:20 with an extraction time of 1 h was best for the majority of elements in the two soils tested. In a limited number of agricultural soils, we were able to show that CHCl3 fumigation extraction can successfully be applied to the elements phosphorus, sulphur, potassium, sodium, magnesium, zinc, manganese, copper, nickel, vanadium, boron, silicon, and barium to yield a CHCl3-labile element fraction. Conversion values to microbial biomass, accounting for elements contained in the cell envelope components, which are mostly not extractable, and to account for adsorption to soil colloids during extraction are yet to be determined in a larger variety of soils. To overcome some of the limitations of the fumigation extraction approach for secondary and trace elements, a pre-extraction step may provide a suitable solution.

中文翻译:

通过熏蒸提取土壤中氯仿不稳定的微量元素:迈向 C:N:P 以外的土壤微生物离子组的步骤

次生元素和微量元素可能会限制土壤微生物的功能,尽管微生物的需求和含量在很大程度上仍然未知,而且它们的原位检测方法也很有限。因此,本研究的目的是朝着开发评估土壤微生物离子组的方法迈出第一步,即土壤微生物群落的元素组成。氯仿(CHCl 3)熏蒸提取用于检测土壤中微生物 CHCl 3不稳定的二次元素和微量元素浓度。两种萃取剂(NH 4 NO 3、CaCl 2 )对 CHCl 3定量的适用性- 不稳定浓度的磷、硫、钾、钠和镁,以及硒、铁、锌、锰、铜、钴、镍、钼、钒、硼、硅、钡、砷和镉,在六个农业土壤。此外,在两种土壤的子集中测试了三种土壤与萃取剂的比例(1:5、1:10 和 1:20)和两种萃取持续时间,1 小时或 2 小时。在测试的两种萃取剂中,发现 0.01 M CaCl 2是最合适的萃取剂。对于 CaCl 2,土壤与萃取剂的比例为 1:20,萃取时间为 1 小时,对于所测试的两种土壤中的大多数元素而言效果最佳。在有限数量的农业土壤中,我们能够证明 CHCl 3熏蒸提取可以成功地应用于元素磷、硫、钾、钠、镁、锌、锰、铜、镍、钒、硼、硅和钡,以产生 CHCl 3 不稳定元素部分。微生物生物量的转换值,占细胞包膜成分中所含的元素,这些元素大多不可提取,并占提取过程中对土壤胶体的吸附,尚未在更多种类的土壤中确定。为了克服二次和痕量元素熏蒸提取方法的一些局限性,预提取步骤可能提供合适的解决方案。
更新日期:2023-02-27
down
wechat
bug