The probiotic Weizmannia coagulans (W. coagulans) BC2000 can increase the abundance of intestinal transforming ellagic acid (EA) bacteria and inhibit metabolic disorders caused by hyperlipidemia by activating liver autophagy. This study aimed to investigate the inhibitory effects of W. coagulans BC2000 and EA on hyperlipidemia-induced cholesterol metabolism disorders. C57BL/6J mice (n = 10 in each group) were fed a low-fat diet, high-fat diet (HFD), HFD supplemented with EA, HFD supplemented with EA and W. coagulans BC77, HFD supplemented with EA, and W. coagulans BC2000. EA and W. coagulans BC2000 supplementation prevented HFD-induced hypercholesterolemia and promoted fecal cholesterol excretion. Transcriptome analysis showed that primary bile acid biosynthesis in the liver was significantly activated by EA and W. coagulans BC2000 treatments. EA and W. coagulans BC2000 treatment also significantly increased the intestinal Eggerthellaceae abundance and the liver EA metabolites, iso-urolithin A, Urolithin A, and Urolithin B. Therefore, W. coagulans BC2000 supplementation promoted the intestinal transformation of EA, which led to the upregulation of liver bile synthesis, thus preventing hypercholesterolemia.

中文翻译：

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Weizmannia coagulans BC2000 和鞣花酸的补充通过促进小鼠肝脏初级胆汁酸生物合成和肠道胆固醇排泄来抑制高脂肪诱导的高胆固醇血症。

益生菌 Weizmannia coagulans (W. coagulans) BC2000 可以增加肠道转化鞣花酸 (EA) 细菌的丰度，并通过激活肝脏自噬来抑制高脂血症引起的代谢紊乱。本研究旨在探讨 W. coagulans BC2000 和 EA 对高脂血症引起的胆固醇代谢紊乱的抑制作用。C57BL/6J 小鼠（每组 n = 10）喂食低脂饮食、高脂饮食 (HFD)、HFD 补充 EA、HFD 补充 EA 和 W. coagulans BC77、HFD 补充 EA 和 W . 凝固剂 BC2000。EA 和 W. coagulans BC2000 补充剂可预防 HFD 诱导的高胆固醇血症并促进粪便中胆固醇的排泄。转录组分析表明，EA 和 W. coagulans BC2000 处理可显着激活肝脏中的初级胆汁酸生物合成。