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Network Pharmacology-Based Identification of Key Targets of Ziyin Mingmu Pills Acting on Age-Related Macular Degeneration
Evidence-based Complementary and Alternative Medicine ( IF 2.650 ) Pub Date : 2023-2-2 , DOI: 10.1155/2023/5933125
Yijing Yang 1, 2 , Ying Wang 1 , Xiaoqing Liu 1 , Ying Deng 1 , Jing Lu 1, 2 , Feipeng Jiang 1 , Fujiao Nie 1 , Jun Peng 1, 3 , Qinghua Peng 1, 2, 3 , Yuhui Qin 1, 2, 3
Affiliation  

Objective. This study is designed to find out the molecular targets of effective Chinese medicine Ziyin Mingmu pills (ZMPs) in treating age-related macular degeneration (AMD) based on network pharmacology and experimental data. Methods. A comprehensive network pharmacology strategy that consists of three sequential modules (drug-disease target molecular docking, enrichment analysis, and external verification) was carried out to identify potential targets of ZMPs acting on AMD. Results. The active ingredients of ZMPs targeting 66 genes have effects on the process of AMD. GO and KEGG pathway enrichment analyses suggested that response to oxidative stress, regulation of angiogenesis, and lipid and atherosclerosis might serve as the most important signaling pathways in ZMPs for AMD treatment. Combined with the GSE29801 dataset for further analysis, two key genes, EGFR and VEGFA, were identified. Immune infiltration analysis showed that there was a strong association between EGFR and immune cell content. In addition, images were acquired following 24 h in the scratch experiment showed that ZMPs can reduce the percentage of wound healing distance. The Western blot assay found that ZMPs increased the expression of EGFR and decreased the expression of VEGFA. Conclusion. This study sheds light on some mechanisms of ZMP therapy for AMD, particularly the effect of ZMP on the oxidative stress in RPE and cell survival and angiogenesis in AMD. We propound ZMPs as a promising strategy to intervene in the process of AMD.

中文翻译:

基于网络药理学的滋阴明目丸作用于老年性黄斑变性关键靶点的鉴定

目标。本研究旨在基于网络药理学和实验数据找出有效中药滋阴明目丸(ZMPs)治疗老年性黄斑变性(AMD)的分子靶点。方法。进行了由三个连续模块(药物-疾病靶点分子对接、富集分析和外部验证)组成的综合网络药理学策略,以确定作用于 AMD 的 ZMP 的潜在靶点。结果. 针对66个基因的ZMPs活性成分对AMD的进程有影响。GO 和 KEGG 通路富集分析表明,对氧化应激的反应、血管生成的调节以及脂质和动脉粥样硬化可能是 ZMP 治疗 AMD 中最重要的信号通路。结合 GSE29801 数据集进行进一步分析,确定了两个关键基因 EGFR 和 VEGFA。免疫浸润分析表明,EGFR 与免疫细胞含量之间存在很强的相关性。此外,在划痕实验中 24 小时后获取的图像显示 ZMP 可以减少伤口愈合距离的百分比。Western blot检测发现ZMPs增加了EGFR的表达,降低了VEGFA的表达。结论. 本研究阐明了 ZMP 治疗 AMD 的一些机制,特别是 ZMP 对 RPE 氧化应激以及 AMD 细胞存活和血管生成的影响。我们提出 ZMPs 作为干预 AMD 过程的有前途的策略。
更新日期:2023-02-02
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