The endocrine system's interference caused by environmental estrogens (EEs) residue in food is a topic of public concern. Here, we construct an aptasensor for the sensitive detection of EEs based on luminescence resonance energy transfer (LRET). With MoS₂ nanosheets acting as the energy acceptor and upconversion luminescence nanoparticles@gold nanoparticles (UCNPs@Au) as the luminescence donor, autofluorescence from food is prevented from interfering. The in-situ deposition of AuNPs not only induces local field enhancement to significantly increase the luminescence intensity of UCNPs, but also conduces to the modification of aptamer through Au-S bond. This aptasensor can respond to multiple estrogens thanks to the choice of a universal aptamer that recognizes phenolic hydroxyl group, and it offers the probability to screen unidentified phenolic estrogens. This method has a high sensitivity and a low limit of detection (LOD), and the satisfactory recovery rates acquired from water and milk samples confirmed its considerable application value.

食品中环境雌激素（EEs）残留对内分泌系统的干扰是公众关注的话题。在这里，我们构建了一个适体传感器，用于基于发光共振能量转移 (LRET) 的 EE 灵敏检测。含_二硫化钼纳米片作为能量受体和上转换发光纳米粒子@金纳米粒子（UCNPs@Au）作为发光供体，可以防止来自食物的自发荧光干扰。AuNPs的原位沉积不仅诱导局部场增强显着增加UCNPs的发光强度，而且有助于通过Au-S键修饰适体。由于选择了识别酚羟基的通用适体，该适体传感器可以响应多种雌激素，并提供了筛选未鉴定酚类雌激素的可能性。该方法具有高灵敏度和低检测限 (LOD)，从水和牛奶样品中获得的令人满意的回收率证实了其相当大的应用价值。