Herein, we report a novel aggregation-induced emission nanoparticles (AIENPs)-based immunochromatography assay (ICA) platform to detect ochratoxin A (OTA) using orange-yellow-emitting AIENPs as fluorescent nanoprobes. Immunochromatographic strip is used for the quantitative detection of OTA in crop matrix using AIENPs coupled with anti-OTA ascites. Under optimal conditions, AIENPs-ICA exhibits stronger signal output capacity and higher sensitivity than traditional gold nanoparticles-based ICA. The half-maximal inhibitory concentration is as low as 0.149 ng mL^-1, and the limit detection is 0.042 ng mL^-1 at 10% competitive inhibition concentration. The average recovery of AIENPs-ICA ranges from 82.60% to 113.14% with the coefficient of variation ranging from 1.26% to 11.57%, proving the proposed method possesses good reliability and reproducibility. Moreover, the developed AIENPs-ICA exhibits negligible cross-reactions with other mycotoxins. We believe the presented AIENPs-ICA platform holds promising potential as a powerful tool for on-site detection of OTA and other molecules detection in food samples.

在此，我们报告了一种新型的基于聚集诱导发射纳米粒子 (AIENPs) 的免疫层析分析 (ICA) 平台，使用橙黄色发光的 AIENPs 作为荧光纳米探针来检测赭曲霉毒素 A (OTA)。免疫层析试纸条用于定量检测作物基质中的 OTA，使用 AIENPs 结合抗 OTA 腹水。在最佳条件下，AIENPs-ICA 表现出比传统的基于金纳米粒子的 ICA 更强的信号输出能力和更高的灵敏度。半数抑菌浓度低至0.149 ng mL ^-1，检测限为0.042 ng mL ^-1在 10% 的竞争抑制浓度下。AIENPs-ICA的平均回收率为82.60%~113.14%，变异系数为1.26%~11.57%，证明该方法具有良好的可靠性和重现性。此外，开发的 AIENPs-ICA 与其他霉菌毒素的交叉反应可忽略不计。我们相信所提出的 AIENPs-ICA 平台作为现场检测食品样品中 OTA 和其他分子检测的强大工具具有广阔的潜力。