This work reports the optimization of a method using Molecularly Imprinted Polymers (MIPs) for the simultaneous determination of zearalenone and alternariol mycotoxins. The method was optimized using a chemometric approach where in the optimized conditions, the cartridges with a mixture (50:50, w/w) of both MIPs, were loaded with 30 mL of sample, washed with 2 mL of ACN/water (20/80, v/v) and eluted with 2.5 mL of trifluoroacetic acid/MeOH (3/97, v/v). The extracts were analyzed by HPLC coupled to a fluorescence detector (FLD). The optimized method has been applied and validated to the analysis of the mycotoxins in maize, sunflower and olive oils samples with a limit of detection of 5 and 2 µg kg^-1, respectively. Recoveries were in the range of 94% to 108% (RSD < 6%) for zearalenone and 92% to 113% (RSD < 5%) for alternariol. The results were confirmed by HPLC-MS/MS.

这项工作报告了一种使用分子印迹聚合物 (MIP) 同时测定玉米赤霉烯酮和交链孢霉毒素的方法的优化。该方法使用化学计量学方法进行了优化，在优化条件下，将两种 MIP 的混合物 (50:50，w/w) 装入 30 mL 样品的小柱，用 2 mL 乙腈/水 (20 /80, v/v) 并用 2.5 mL 三氟乙酸/MeOH (3/97, v/v) 洗脱。通过与荧光检测器 (FLD) 耦合的 HPLC 分析提取物。优化后的方法已应用于玉米、向日葵和橄榄油样品中霉菌毒素的分析并得到验证，检测限为 5 和 2 µg kg ^-1， 分别。玉米赤霉烯酮的回收率在 94% 到 108%（RSD < 6%）之间，交链孢酚的回收率在 92% 到 113%（RSD < 5%）之间。结果通过 HPLC-MS/MS 确认。