As kanamycin (KAN) residue in animal products is harmful to consumers, a rapid and sensitive method for KAN detection needs to be established. KAN monoclonal antibody (KAN-mAb, 1D11) with the half maximal inhibitory concentration of 1.16 ng/mL was prepared in this study. A one-pot method was used to synthesize Fe³⁺-chelated polydopamine nanospheres (Fe@PDANs) with excellent characteristics of strong light absorption. The novel label of Fe@PDANs and KAN-mAb was used to develop a lateral flow immunoassay (LFIA) for the sensitive detection of KAN. The limit of detection of the Fe@PDANs-based LFIA (Fe@PDANs-LFIA) for KAN was 0.0191 ng/mL, which was 2.75 times lower than PDANs-based LFIA. Furthermore, the Fe@PDANs-LFIA was successfully applied to detect KAN in pork, milk, and honey samples, with recoveries ranging from 93.75% to 113.80% (coefficient of variation <10%). Therefore, Fe@PDANs have potential for the detection of analytes in LFIA.

由于动物产品中的卡那霉素 (KAN) 残留对消费者有害，因此需要建立一种快速、灵敏的 KAN 检测方法。本研究制备了半数最大抑制浓度为 1.16 ng/mL 的 KAN 单克隆抗体 (KAN-mAb, 1D11)。一锅法合成Fe ³⁺-螯合聚多巴胺纳米球（Fe@PDANs）具有优异的强光吸收特性。Fe@PDANs 和 KAN-mAb 的新型标签被用于开发一种侧流免疫分析 (LFIA) 以灵敏检测 KAN。基于 Fe@PDANs 的 LFIA（Fe@PDANs-LFIA）对 KAN 的检测限为 0.0191 ng/mL，比基于 PDANs 的 LFIA 低 2.75 倍。此外，Fe@PDANs-LFIA 成功应用于检测猪肉、牛奶和蜂蜜样品中的 KAN，回收率在 93.75% 到 113.80% 之间（变异系数 <10%）。因此，Fe@PDANs 具有检测 LFIA 中分析物的潜力。