人巨细胞病毒编码的 microRNAs 在主动脉夹层患者血浆中的表达谱,Journal of Cardiothoracic Surgery

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人巨细胞病毒编码的 microRNAs 在主动脉夹层患者血浆中的表达谱
Journal of Cardiothoracic Surgery ( IF 1.6 ) Pub Date : 2023-01-18 , DOI: 10.1186/s13019-023-02122-7
Yongqing Cheng ₁ , Yufan Du ₂ , Qi Wang ₂ , Qinghe Lv ₂ , Yunxin Xue ₁ , Weihong Zhou ₃ , Chenyu Zhang ₂ , Xi Chen ₂ , Dongjin Wang ₁

Affiliation

主动脉夹层 (AD) 是一种罕见的疾病，死亡率很高，目前尚无有效的诊断生物标志物。人巨细胞病毒（human cytomegalovirus，HCMV）感染是许多疾病发生和发展的重要原因，但HCMV感染与AD的关系尚不明确。在这项研究中，我们首先使用定量逆转录聚合酶链反应 (qRT-PCR) 来确定由 20 名 AD 患者和 20 名健康对照组成的训练集中血浆中 25 种 HCMV 编码的 microRNA (HCMV miRNA) 的表达谱。然后，异常表达的 HCMV miRNA 在 12 名 AD 患者和 12 名健康对照的验证集中得到验证。此外，在由 20 名 AD 患者和 20 名健康对照组成的第三组中检测到 HCMV 感染。训练集中 HCMV miRNA 表达水平的 95% 分位数用作区分 AD 患者和健康对照的阈值。五种HCMV miRNA水平高的个体比例在AD患者和健康对照之间存在显着差异。在验证集中，只有 hcmv-miR-UL112-5p 和 hcmv-miR-UL22A-5p（初步筛选中获得的五个 HCMV miRNA 中的两个）高水平的个体比例显示 AD 患者与健康人之间存在显着差异控制。在第三组中，AD 患者和健康对照之间的 HCMV DNA 水平和抗 HCMV IgG 浓度没有显着差异。AD 患者和健康对照者血浆中的 HCMV miRNA 水平不同。

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Human cytomegalovirus-encoded microRNAs expression profile in plasma of patients with aortic dissection

Aortic dissection (AD) is a rare disease with high mortality for which no effective diagnostic biomarkers are available. Human cytomegalovirus (HCMV) infection is an important cause of the occurrence and progression of many diseases, but the relationship between HCMV infection and AD is not clear. In this study, we first used quantitative reverse transcription polymerase chain reaction (qRT-PCR) to determine the expression profile of 25 HCMV-encoded microRNAs (HCMV miRNAs) in the plasma within a training set consisting of 20 AD patients and 20 healthy controls. Then, abnormal expressed HCMV miRNAs were verified in a validation set of 12 AD patients and 12 healthy controls. In addition, HCMV infection was detected in the third cohort consisting of 20 AD patients and 20 healthy controls. The 95% quantile of the expression levels of HCMV miRNAs in the training set was used as the threshold for distinction between AD patients and healthy controls. The proportion of individuals with high level of five types of HCMV miRNAs was significantly different between AD patients and healthy controls. In the validation set, only the proportion of individuals with high levels of hcmv-miR-UL112-5p and hcmv-miR-UL22A-5p, two of the five HCMV miRNAs obtained in the preliminary screening, showed significant difference between AD patients and healthy controls. In the third cohort, there was no significant difference in HCMV DNA levels and anti-HCMV IgG concentrations between AD patients and healthy controls. The HCMV miRNAs levels in plasma differed in AD patients and healthy controls. This finding may contribute to a further understanding of the relationship between HCMV infection and AD and are worthy of future research on the diagnosis and etiology of AD.

更新日期：2023-01-19

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