Regeneration of injured articular cartilage is limited by low early-stage recruitment of stem cells and insufficient chondrogenic differentiation. Hydrogels are widely used to repair cartilage because they have excellent mechanical and biological properties. In this study, a dual drug-loaded thermosensitive hydroxypropyl chitin hydrogel (HPCH) system was prepared to release stromal-derived factor-1α-like polypeptides (SDFP) and kartogenin (KGN) for stem-cell recruitment and chondrogenic differentiation. The hydrogel had a network structure that promoted cell growth and nutrient exchange. Moreover, it was temperature sensitive and suitable for filling irregular defects. The system showed good biocompatibility in vitro and promoted stem-cell recruitment and chondrogenic differentiation. Furthermore, it reduced chondrocyte catabolism under inflammatory conditions. Animal experiments demonstrated that the dual-drug hydrogel systems can promote the regeneration of articular cartilage in rats. This study confirmed that an HPCH system loaded with KGN and SDFP could effectively repair articular cartilage defects and represents a viable treatment strategy.

受损关节软骨的再生受到早期干细胞募集低和软骨分化不足的限制。水凝胶被广泛用于修复软骨，因为它们具有优异的机械和生物学特性。在这项研究中，制备了一种双载药热敏羟丙基甲壳素水凝胶 (HPCH) 系统，以释放基质衍生因子 1α 样多肽 (SDFP) 和 kartogenin (KGN)，用于干细胞募集和软骨形成分化。水凝胶具有促进细胞生长和营养交换的网络结构。此外，它对温度敏感，适用于填充不规则缺陷。该系统在体外表现出良好的生物相容性，促进干细胞募集和软骨分化。此外，它减少了炎症条件下的软骨细胞分解代谢。动物实验表明，双药水凝胶系统可以促进大鼠关节软骨的再生。该研究证实，装载 KGN 和 SDFP 的 HPCH 系统可以有效修复关节软骨缺损，是一种可行的治疗策略。