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Bilberry Anthocyanins (Vaccinium myrtillus L.) Induced Apoptosis of B16-F10 Cells and Diminished the Effect of Dacarbazine
Nutrition and Cancer ( IF 2.9 ) Pub Date : 2022-12-19 , DOI: 10.1080/01635581.2022.2157450
Xuehong Yu 1 , Yuhua Yue 2 , Haizhu Shi 1 , Kaiyan Xu 1 , Chungu Zhang 1 , Yu Wan 1 , Shun Feng 1
Affiliation  

Abstract

Anthocyanins have been reported to have potential as dietary or pharmaceutical supplements in the application of cancer prevention and adjunctive treatment. However, there are few studies on the effect of anthocyanins on melanoma, which have only been performed in cell lines. The objective of this work was to investigate the anticancer effects and mechanisms of bilberry anthocyanin extract (BAE) on melanoma In Vitro and In Vivo. Moreover, a primary study was done to investigate how BAE influenced C57BL/6 mice bearing subcutaneous B16-F10 tumors treated with dacarbazine (DTIC). BAE-induced apoptosis in B16-F10 cells was associated with activation of the mitochondrial pathway induced by increased reactive oxygen species. More, In Vivo anticancer activity studies indicated that BAE attenuated melanoma growth, as identified by hematoxylin-eosin staining, Ki-67, and TUNEL assays. Further western blot results revealed higher phospho-Akt expression with the combination of BAE and DTIC, indicating no suppression of the PI3K/AKT signaling pathway. In summary, this study demonstrated the anti-melanoma activity of BAE and investigated its mechanism. Notably, it should be careful to use products enriching BAE for those melanoma patients treated with DTIC.



中文翻译:

越橘花青素 (Vaccinium myrtillus L.) 诱导 B16-F10 细胞凋亡并减弱达卡巴嗪的作用

摘要

据报道,花青素在癌症预防和辅助治疗的应用中具有作为膳食或药物补充剂的潜力。然而,关于花青素对黑色素瘤影响的研究很少,仅在细胞系中进行过。这项工作的目的是研究越橘花青素提取物 (BAE) 在体外和体内对黑色素瘤的抗癌作用和机制。此外,还进行了一项初步研究,以研究 BAE 如何影响携带用达卡巴嗪 (DTIC) 治疗的皮下 B16-F10 肿瘤的 C57BL/6 小鼠。BAE 诱导的 B16-F10 细胞凋亡与活性氧增加诱导的线粒体通路激活有关。此外,体内抗癌活性研究表明 BAE 减弱了黑色素瘤的生长,通过苏木精-伊红染色、Ki-67 和 TUNEL 测定法鉴定。进一步的蛋白质印迹结果显示,BAE 和 DTIC 的组合具有更高的磷酸化 Akt 表达,表明没有抑制 PI3K/AKT 信号通路。总之,本研究证明了 BAE 的抗黑色素瘤活性并研究了其机制。值得注意的是,对于接受 DTIC 治疗的黑色素瘤患者,应谨慎使用富含 BAE 的产品。

更新日期:2022-12-19
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