Although lipids are not genetically encoded, they are fundamental building blocks of cell membranes and essential components of cell metabolites. Lipids regulate various biological processes, including energy storage, membrane trafficking, signal transduction, and protein secretion; therefore, their metabolic imbalances cause many diseases. Approximately 47 000 lipid species with diverse structures have been identified, but little is known about their crucial roles in cellular systems. Particularly the structural, metabolic, and signaling functions of lipids often arise from interactions with proteins. Lipids attach to proteins not only by covalent bonds but also through noncovalent interactions, which also influence protein functions and localization. Therefore, it is important to explore this lipid–protein “interactome” to understand its roles in health and disease, which may further provide insight for medicinal development. However, lipid structures are generally quite complicated, rendering the systematic characterization of lipid–protein interactions much more challenging.

中文翻译：

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双功能脂质衍生亲和探针 (AfBPs) 用于分析脂质-蛋白质相互作用组

虽然脂质不是基因编码的，但它们是细胞膜的基本组成部分和细胞代谢物的基本成分。脂质调节各种生物过程，包括能量储存、膜运输、信号转导和蛋白质分泌；因此，他们的代谢失衡会导致许多疾病。已鉴定出大约 47000 种具有不同结构的脂质种类，但对其在细胞系统中的关键作用知之甚少。特别是脂质的结构、代谢和信号功能通常来自与蛋白质的相互作用。脂质不仅通过共价键而且通过非共价相互作用与蛋白质结合，这也影响蛋白质的功能和定位。所以，探索这种脂质-蛋白质“相互作用组”以了解其在健康和疾病中的作用非常重要，这可能进一步为药物开发提供见解。然而，脂质结构通常相当复杂，使得脂质-蛋白质相互作用的系统表征更具挑战性。