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Design and Synthesis of Novel Fluorescent Probe Based on Cyanobiphenyl and its Application in Detection of Hypochlorite
Journal of Fluorescence ( IF 2.7 ) Pub Date : 2022-12-01 , DOI: 10.1007/s10895-022-03094-y
Boxin Zhou 1 , Yunlong Han 1 , Jingyu Liu 2 , Kai Cheng 1 , Mingdong Dong 1 , Xu Tang 1
Affiliation  

Hypochlorite is an important biological reactive oxygen species, which plays a pivotal role in various life activities. Excessive presence in the human body or excessive intake in life causes a series of diseases. To monitor the hypochlorite level in living cells, organisms and environment water samples, we herein designed and synthesized three organic small molecule fluorescent probes with different recognition sites based on nitrile biphenyl. Through performance comparison, it was found that probe A-HM exhibited the best detection performance for hypochlorite with a low detection limit of 2.47 × 10–6 M. The introduction of hypochlorite will induce probe fluorescence A-HM to turn on, and the fluorescence colour will change from colourless to green. The application of A-HM in biological systems has been demonstrated by the imaging monitoring of hypochlorite in MCF-7, L929 cells and zebrafish. Furthermore, A-HM was also used for the accurate determination of the hypochlorite level in real water samples with high sensitivity and good recoveries.



中文翻译:

基于氰基联苯的新型荧光探针的设计合成及其在次氯酸盐检测中的应用

次氯酸盐是一种重要的生物活性氧,在各种生命活动中起着举足轻重的作用。在人体内过量存在或生活中摄入过多会引起一系列疾病。为了监测活细胞、生物体和环境水样中的次氯酸盐水平,我们在此设计并合成了三种基于腈联苯的具有不同识别位点的有机小分子荧光探针。通过性能比较发现,探针A-HM对次氯酸盐的检测性能最好,检出限低至2.47×10 –6 M.次氯酸盐的引入会诱导探针荧光A-HM开启,荧光颜色由无色变为绿色。A-HM 在生物系统中的应用已通过 MCF-7、L929 细胞和斑马鱼中次氯酸盐的成像监测得到证实。此外,A-HM 还用于准确测定实际水样中的次氯酸盐水平,具有高灵敏度和良好的回收率。

更新日期:2022-12-01
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