Fluorescence laser-scanning microscopy (LSM) is experiencing a revolution thanks to new single-photon (SP) array detectors, which give access to an entirely new set of single-photon information. Together with the blooming of new SP LSM techniques and the development of tailored SP array detectors, there is a growing need for (i) DAQ systems capable of handling the high-throughput and high-resolution photon information generated by these detectors, and (ii) incorporating these DAQ protocols in existing fluorescence LSMs. We developed an open-source, low-cost, multi-channel time-tagging module (TTM) based on a field-programmable gate array that can tag in parallel multiple single-photon events, with 30 ps precision, and multiple synchronisation events, with 4 ns precision. We use the TTM to demonstrate live-cell super-resolved fluorescence lifetime image scanning microscopy and fluorescence lifetime fluctuation spectroscopy. We expect that our BrightEyes-TTM will support the microscopy community in spreading SP-LSM in many life science laboratories.

荧光激光扫描显微镜 (LSM) 正在经历一场革命，这要归功于新的单光子 (SP) 阵列探测器，它可以访问一组全新的单光子信息。随着新 SP LSM 技术的蓬勃发展和定制 SP 阵列探测器的发展，人们越来越需要 (i) 能够处理这些探测器产生的高通量和高分辨率光子信息的 DAQ 系统，以及 (ii) ) 将这些 DAQ 协议合并到现有的荧光 LSM 中。我们基于现场可编程门阵列开发了一种开源、低成本、多通道时间标记模块 (TTM)，可以并行标记多个单光子事件，精度为 30 ps，以及多个同步事件，精度为 4 ns。我们使用 TTM 来展示活细胞超分辨荧光寿命图像扫描显微镜和荧光寿命波动光谱。我们期望我们的 BrightEyes-TTM 将支持显微镜界在许多生命科学实验室中传播 SP-LSM。