Identification of cis/trans-carbon-carbon double-bond (C=C) isomers remain challenging. Herein, a simple and rapid method for the separation and analysis of cis/trans-maleic acid (MA) and aconitic acid (AA) using Trapped Ion Mobility Spectrometry (TIMS) was developed. α-, β-, γ-cyclodextrin (CD) were served as the separation reagent, slight difference in mobility separation was obtained by [CD-MA/AA-H]^-. Specially, with the addition of divalent metal ion (G²⁺) as coordination metal ion, the separation effect was much increased by [CD-MA/AA+G-H]⁺, and α-CD has better mobility separation effect than β-/γ-CD. Moreover, chemical calculations revealed the binary and ternary complexes are in the inclusion forms, and microscopic interactions between cis/trans-MA/AA, CDs, and G²⁺ are somewhat different that making their mobility separation. Finally, quantifications of cis/trans-isomers were analyzed in food samples, with good linearity (R²>0.99) and recoveries obtained from 87.25% to 100.73%.

顺式/反式碳碳双键 (C=C) 异构体的鉴定仍然具有挑战性。在此，开发了一种使用俘获离子淌度谱 (TIMS) 分离和分析顺/反马来酸 (MA) 和乌头酸 (AA) 的简单快速方法。以α-、β-、γ-环糊精(CD)为分离试剂，[CD-MA/AA-H] ^-分离的迁移率略有差异。特别地，加入二价金属离子（G ^{2+ ）作为配位金属离子后，[CD-MA/AA+GH] +}的分离效果大大提高, α-CD 的迁移率分离效果优于 β-/γ-CD。此外，化学计算表明二元和三元配合物呈包合物形式，cis/trans-MA/AA、CDs和G ²⁺之间的微观相互作用有所不同，导致它们的迁移率分离。最后，对食品样品中的顺式/反式异构体进行定量分析，线性良好（R ² > 0.99），回收率为 87.25% 至 100.73%。