Adenosine triphosphate (ATP) provides the driving force necessary for critical biological functions in all living organisms. In synthetic biocatalytic reactions, this cofactor is recycled in situ using high-energy stoichiometric reagents, an approach that generates waste and poses challenges with enzyme stability. On the other hand, an electrochemical recycling system would use electrons as a convenient source of energy. We report a method that uses electricity to turn over enzymes for ATP generation in a simplified cellular respiration mimic. The method is simple, robust, and scalable, as well as broadly applicable to complex enzymatic processes including a four-enzyme biocatalytic cascade in the synthesis of the antiviral molnupiravir.

中文翻译：

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生物催化反应级联中三磷酸腺苷的电化学回收

三磷酸腺苷 (ATP) 为所有生物体的关键生物学功能提供必要的驱动力。在合成生物催化反应中，这种辅助因子使用高能化学计量试剂原位回收，这种方法会产生废物并对酶的稳定性提出挑战。另一方面，电化学回收系统将使用电子作为方便的能源。我们报告了一种在简化的细胞呼吸模拟中使用电来转换酶以生成 ATP 的方法。该方法简单、稳健且可扩展，并且广泛适用于复杂的酶促过程，包括抗病毒药物 molnupiravir 合成中的四酶生物催化级联。