It is challenging to achieve the highly sensitive detection of glucocorticoids at ultratrace levels because of the abundant hydrophilic groups in their molecules and the complexity of environmental water sample matrices. Here, a highly crystalline three-dimensional hydroxylated covalent organic frameworks (denoted by COF-301) with tetra(4-anilyl)methane (TAM) and 2,5-dihydroxyterephthalaldehyde (DHTA) as building units was constructed and proposed as adsorbent for solid phase extraction (SPE) of glucocorticoids. Theoretical studies were conducted to elucidate the potential adsorption mechanism of glucocorticoids on the COF-301. The COF-301 based SPE combined with liquid chromatography-tandem mass spectrometry provides a promising approach for the preconcentration and determination of glucocorticoids residue in water samples. Good linearity with a correlation coefficient exceeding 0.9988, low limits of detection ranging from 0.024 to 0.075 ng L⁻¹ and relative standard deviations below 6.68% were achieved. The proposed method was successfully applied to analyze glucocorticoids residue in actual water samples, demonstrating the prospects of this method for the determination of trace glucocorticoids.

由于其分子中丰富的亲水基团和环境水样基质的复杂性，实现超痕量水平糖皮质激素的高灵敏度检测具有挑战性。在这里，构建了以四（4-苯胺基）甲烷（TAM）和 2,5-二羟基对苯二甲醛（DHTA）为结构单元的高度结晶的三维羟基化共价有机框架（由 COF-301 表示），并建议将其用作固体吸附剂糖皮质激素的相萃取 (SPE)。进行了理论研究以阐明糖皮质激素在 COF-301 上的潜在吸附机制。基于 COF-301 的固相萃取与液相色谱-串联质谱联用为水样中糖皮质激素残留的预浓缩和测定提供了一种很有前途的方法。^-1且相对标准偏差低于 6.68%。该方法成功应用于实际水样中糖皮质激素残留量的分析，展示了该方法用于痕量糖皮质激素测定的前景。