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Concentration, chemical composition and toxicological responses of the ultrafine fraction of urban air particles in PM1
Environment International ( IF 11.8 ) Pub Date : 2022-11-28 , DOI: 10.1016/j.envint.2022.107661
Mo Yang, Qi-Zhen Wu, Yun-Ting Zhang, Ari Leskinen, Mika Komppula, Henri Hakkarainen, Marjut Roponen, Shu-Li Xu, Li-Zi Lin, Ru-Qing Liu, Li-Wen Hu, Bo-Yi Yang, Xiao-Wen Zeng, Guang-Hui Dong, Pasi Jalava

Epidemiological and toxicological evidence show that PM1 is a key driver of PM2.5. However, the particle sizes that play the major damaging role in PM1 is unclear. Our aims were to 1) explore the concentration and chemical composition of ultrafine particles in PM1; 2) evaluate and compare the differences in toxicity of PM1-0.2 and PM0.2 to different airway cell models. We collected PM1-0.2 and PM0.2 in Guangzhou in December 2017, monitored and detected their concentrations, size, and components. A549, THP-1 mono-cultures and A549/THP-1 co-cultured cells were exposed to PM1-0.2 and PM0.2, and evaluated for toxicological responses. PM0.2 contributed to15% PM1 mass and 87.67% to particle number (PN). Most of PM0.2 was PM0.1 (exceed 75%). Structure of particles in PM0.2 were mostly spherical, whereas in PM1-0.2 they were more diverse. Within the same mass unit, PM0.2 contained more chemical components than PM1-0.2; within the same volume, a higher mass of components was found in PM1-0.2. IL-6 and IL-8 were secreted by co-cultured cells in higher manner compared to mono cell cultures. PM0.2-induced higher toxicological responses compared to PM1-0.2. Our findings suggest that high PN concentration and toxicity of ultrafine or PM1 cannot be ignored. Mono -cell culture may underestimate the true PM toxicity.



中文翻译:

PM1 中城市空气颗粒物超细部分的浓度、化学成分和毒理学反应

流行病学和毒理学证据表明,PM 1是 PM 2.5的关键驱动因素。然而,在 PM 1中起主要破坏作用的颗粒大小尚不清楚。我们的目标是 1) 探索 PM 1中超细颗粒物的浓度和化学成分;2) 评估和比较PM 1-0.2和PM 0.2对不同气道细胞模型的毒性差异。我们于2017年12月在广州采集了PM 1-0.2和PM 0.2,对其浓度、大小和成分进行了监测和检测。A549、THP-1 单一培养物和 A549/THP-1 共培养细胞暴露于 PM 1-0.2和 PM 0.2,并评估了毒理学反应。PM 0.2贡献了 15% 的 PM 1质量和 87.67% 的粒子数 (PN)。PM 0.2的大部分是 PM 0.1(超过 75%)。PM 0.2中的颗粒结构主要为球形,而 PM 1-0.2中的颗粒结构更加多样化。在相同质量单位内,PM 0.2所含化学成分多于PM 1-0.2;在相同的体积内,在 PM 1-0.2中发现了更高质量的组分。与单细胞培养物相比,共培养细胞以更高的方式分泌 IL-6 和 IL-8。与 PM 1-0.2相比,PM 0.2引起更高的毒理学反应. 我们的研究结果表明,超细颗粒物或 PM 1的高 PN 浓度和毒性不容忽视。单细胞培养可能低估了真正的 PM 毒性。

更新日期:2022-11-28
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