The heartwood of Syringa oblata Lindl. (SO) is one of Mongolian folk medicines to treat insomnia and pain, while its pharmacological evaluation and underlying mechanism remain unclear. In this study, the sedative effect of ethanol extract of SO (ESO) was evaluated with the locomotor activity test and the threshold dose of pentobarbital sodium-induced sleep test in mice, and the hot plate test, acetic acid-induced writhing test, and formalin test in mice were used to evaluate its analgesic effect. The underlying mechanism of ESO analgesia was explored by RT-PCR and western blot analysis, which is associated with the regulation of the NF-κB signaling pathway. Besides, the main constituents of ESO were characterized by LC/MS data analysis and comparison with isolated pure compounds. The current findings brought evidence for clinical application and further pharmacological and phytochemical studies on SO.

中文翻译：

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含主要木酚素类的蒙古族民间药紫丁香心材乙醇提取物对小鼠镇痛镇静作用

紫丁香的心材林德尔。(SO) 是蒙古族治疗失眠和疼痛的民间药物之一，但其药理评价和潜在机制尚不清楚。本研究通过小鼠运动活性试验和阈剂量戊巴比妥钠诱导睡眠试验、热板试验、醋酸扭体试验、用小鼠福尔马林试验评价其镇痛作用。通过 RT-PCR 和蛋白质印迹分析探讨了 ESO 镇痛的潜在机制，这与 NF-κB 信号通路的调节有关。此外，ESO 的主要成分通过 LC/MS 数据分析和与分离的纯化合物的比较进行了表征。