Numerous micro-sized artificial turf fragments (MATF) and rubber particles (MRP) are generated and accumulated during the use of the artificial playing field. However, attention has rarely been paid to the potential toxic effects of MATF and MRP on sportsmen. In this study, the active components and chemical composition of aged MATF and MRP derived from laboratory photoaging and actual field sampling were detected, and their effects on cytotoxicity were examined correspondingly. Laboratory photoaging significantly increased environmental persistent free radicals (EPFRs), reactive oxygen species (ROS) abundances and oxidative potential (OP) levels on MATF and MRP, but they have limited cytotoxicity. Unfortunately, in the actual field, aged MATF and MRP with higher heavy metals and polycyclic aromatic hydrocarbons (PAHs) contents exhibited markedly higher cytotoxicity with the survival rate of cells of 78% and 26% (p < 0.05), although they had lower EPFRs and ROS yields. Correlation analysis revealed that the cell viability was closely linked to heavy metals of MATF (p < 0.05), and to organic hydroperoxide (OHP), PAHs and heavy metals of MRP (p < 0.05). By systematically considering the above results, heavy metals and PAHs enriched on MATF and MRP from the surrounding environment played the important role in the cytotoxicity, which was different from conventional perspectives. Our findings demonstrate that MATF and MRP associated with an artificial turf field contain potent mixtures of pollutants and can, therefore, be relevant yet underestimated factors contributing to the health risks.

在人造运动场的使用过程中，会产生并积累无数微小的人造草坪碎片（MATF）和橡胶颗粒（MRP）。然而，很少有人关注 MATF 和 MRP 对运动员的潜在毒性作用。本研究对实验室光老化和实际野外采样得到的老化MATF和MRP的活性成分和化学成分进行了检测，并相应考察了它们对细胞毒性的影响。实验室光老化显着增加了 MATF 和 MRP 上的环境持久性自由基 (EPFR)、活性氧 (ROS) 丰度和氧化电位 (OP) 水平，但它们的细胞毒性有限。不幸的是，在实际领域中，p < 0.05)，尽管它们的 EPFR 和 ROS 产量较低。相关性分析表明，细胞活力与 MATF 的重金属 ( p < 0.05) 以及有机氢过氧化物 (OHP)、多环芳烃和 MRP 的重金属 ( p < 0.05) 密切相关。通过系统地考虑上述结果，从周围环境中富集在 MATF 和 MRP 上的重金属和 PAHs 在细胞毒性中起着重要作用，这与传统观点不同。我们的研究结果表明，与人造草坪场地相关的 MATF 和 MRP 含有强效污染物混合物，因此可能是导致健康风险的相关但被低估的因素。