Selenium containing tetraphenyl substituted BODIPY probe was successfully synthesized from respective selenium aldehyde and tetraphenyl pyrrole using Knoevenagel-type condensation. The product was characterized using various spectroscopic techniques (¹ H, ¹³ C, ⁷⁷Se, ¹¹B, and ¹⁹ F) and mass spectrometry. The probe was found to be selective and sensitive towards detection of superoxide over other ROS with a “turn-off” (quenched) fluorescence response. The detection limit of the probe was found to be 4.87 µM. The probe reacted with superoxide in less than a sec with a stoke shift of 35 nm.

中文翻译：

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四苯基取代 BODIPY 基苯基单硒化物探针的合成及光物理研究，用于超氧化物的选择性检测

使用 Knoevenagel 型缩合从各自的硒醛和四苯基吡咯成功合成了含硒四苯基取代的 BODIPY 探针。使用各种光谱技术（¹  H、¹³  C、⁷⁷ Se、¹¹ B 和¹⁹  F）和质谱法对产物进行表征。发现该探针比其他 ROS 具有“关闭”（淬灭）荧光响应，对检测超氧化物具有选择性和敏感性。发现探针的检测限为 4.87 µM。探针在不到 1 秒的时间内与超氧化物反应，冲程位移为 35 nm。