Twelve new clerodane diterpenoids named callicarpanes A–L (1–12), together with eight known compounds (13–20), were isolated from Callicarpa integerrima. Their structures were determined by comprehensive spectroscopic data. The calculated chemical shifts were used to identify relative configurations using DP4+ analysis. The absolute configurations (AC) were assigned based on quantum chemical calculations and X-ray single-crystal diffraction methods. Compounds 1, 3, 5, 9, 10, 12, 15, 16, and 19 showed significant inhibitory activity for NLRP3 inflammasome activation, with the IC₅₀ against lactate dehydrogenase (LDH) release ranging from 0.08 to 4.78 μM. Further study revealed that compound 10 repressed IL-1β secretion and caspase-1 maturation in J774A.1 cell as well as blocked macrophage pyroptosis.

中文翻译：

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Callicarpanes A–L，十二种新的 Clerodane 二萜类化合物，具有来自 Callicarpa integerrima 的 NLRP3 炎症小体抑制活性

从Callicarpa integerrima中分离出12 种名为 callicarpanes A–L ( 1 – 12 )的新的 clerodane 二萜类化合物，以及八种已知化合物 ( 13 – 20 ) 。它们的结构由综合光谱数据确定。计算的化学位移用于使用 DP4+ 分析识别相对构型。绝对构型 (AC) 是根据量子化学计算和 X 射线单晶衍射方法分配的。化合物 1、3、5、9、10、12、15、16和_ _ _ _ _ _ _ _ _ _ _ _ _ _19显示出对 NLRP3 炎性体激活的显着抑制活性，IC ₅₀对乳酸脱氢酶 (LDH) 释放的影响范围为 0.08 至 4.78 μM。进一步研究表明，化合物10抑制 J774A.1 细胞中 IL-1 β的分泌和 caspase-1 的成熟，并阻断巨噬细胞焦亡。