The reaction efficiency of surface-based DNA walker can directly affect the properties of a biosensor. Herein, three-dimensional (3D) DNAzyme walker were first fixed on the top of DNA tetrahedral scaffold to improve the immobilization efficiency. Ferrocene (Fc) that labeled at substrate strand ends effectively quenched the electrochemiluminescence (ECL) signal of Ru(bpy)₂(cpaphen)²⁺, yielding the sensor in a “signal-off” state. Upon the addition of aflatoxin B₁ (AFB₁), 3D DNAzyme walker was activated and fueled by Na⁺, accordingly releasing Fc and recovering the ECL signal of Ru(bpy)₂(cpaphen)²⁺. Due to the high movement efficiency of such 3D DNAzyme walker, ultrasensitive detection of AFB₁ was achieved in the range of 1.0 fg mL⁻¹–10 ng mL⁻¹, with a detection limit of 0.58 fg mL⁻¹. Moreover, satisfactory results were obtained while detecting AFB₁ in corn and peanut samples, suggesting it has a potential application in food safety analysis.

基于表面的 DNA 步行器的反应效率可以直接影响生物传感器的性能。在此，三维 (3D) DNAzyme 步行器首先固定在 DNA 四面体支架的顶部，以提高固定效率。在底物链末端标记的二茂铁 (Fc) 有效地淬灭了 Ru(bpy) ₂ (cpaphen) ²⁺的电化学发光 (ECL) 信号，使传感器处于“信号关闭”状态。添加黄曲霉毒素 B ₁ (AFB ₁ ) 后，3D DNAzyme walker 被激活并由 Na ⁺提供燃料，相应地释放 Fc 并恢复 Ru(bpy) ₂ (cpaphen) ^{2+的 ECL 信号}. 由于这种 3D DNAzyme walker 的高运动效率，AFB ₁的超灵敏检测在 1.0 fg mL ^-1 –10 ng mL ^-1的范围内实现，检测限为 0.58 fg mL ^-1。_{此外，在检测玉米和花生样品中的AFB 1}时获得了令人满意的结果，表明其在食品安全分析中具有潜在的应用价值。