Protein assays with fingerprints and high sensitivity are essential for biomedical research and applications. However, the prevailing methods mainly rely on indirect or labeled immunoassays, failing to provide fingerprint information. Herein, we report a dual biomimetic recognition-driven plasmonic nanogap-enhanced Raman scattering (DBR-PNERS) strategy for ultrasensitive protein fingerprinting and quantitation. A pair of molecularly imprinted nanoantennas were rationally engineered for specifically trapping a target protein into well-defined plasmonic nanogaps through dual-terminal recognition for ultrahigh Raman signal amplification. Meanwhile, a Raman-active small molecule was embedded into the nanoantenna as an internal standard to provide a ratiometric assay for robust quantitation. DBR-PNERS exhibited several significant merits over existing approaches, including fingerprinting, ultrahigh sensitivity, quantitation robustness, speed, sample consumption, and so on. Therefore, it can be a promising tool for a protein assay and holds a great perspective in important applications.

中文翻译：

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双仿生识别驱动等离子体纳米间隙增强拉曼散射用于超灵敏蛋白质指纹图谱和定量

具有指纹图谱和高灵敏度的蛋白质检测对于生物医学研究和应用至关重要。然而，目前流行的方法主要依赖于间接或标记免疫测定，无法提供指纹信息。在此，我们报告了一种用于超灵敏蛋白质指纹识别和定量的双重仿生识别驱动的等离子体纳米间隙增强拉曼散射 (DBR-PNERS) 策略。一对分子印迹纳米天线经过合理设计，通过双端识别超高拉曼信号放大，将目标蛋白特异性捕获到明确的等离子体纳米间隙中。同时，将具有拉曼活性的小分子作为内标嵌入到纳米天线中，以提供用于稳健定量的比率测定。DBR-PNERS 展示了与现有方法相比的几个重要优点，包括指纹识别、超高灵敏度、定量稳健性、速度、样品消耗等。因此，它可以成为蛋白质测定的有前途的工具，并在重要应用中具有广阔的前景。