Introduction and methodsAs a crucial antioxidant enzyme, catalase (CAT) could destroy the cellular hydrogen peroxide to mitigate oxidative stress. The current study aimed to investigate the effects of maternal CAT supplementation from late gestation to day 14 of lactation on antioxidant ability and fatty acids metabolism with regard to the sow-piglet-axis. On day 95 of gestation, forty sows were divided into control (CON) group (fed a basal diet) and CAT group (fed a basal diet supplemented with 660 mg/kg CAT), the feeding experiment ended on day 14 of lactation.ResultsThe lactating sows in the CAT group produced more milk, and had higher antioxidant enzymes activity including POD and GSH-Px (P < 0.05), lower content of serum LDL as well as plasmic C18:3n3 content (P < 0.05). Additionally, maternal CAT supplementation improved offspring's body weight at day 14 of nursing period and ADG (P < 0.05), and regulated the antioxidant ability as evidenced by decreased related enzymes activity such as T-AOC and CAT and changed genes expression level. It significantly affected lipid metabolism of suckling piglets manifested by increasing the serum ALT, CHOL, and LDL (P < 0.05) level and modulating plasma medium- and long-chain fatty acids (MCFAs and LCFAs), as well as regulating the genes expression involved in lipid metabolism.ConclusionMaternal CAT supplementation could regulate the fatty acid composition and enhance the antioxidant ability of sows and offspring during the lactating period and further promote the growth of suckling piglets. These findings might provide a reference value for the utilization of CAT as supplement for mother from late pregnancy to lactation period to promote the fatty acid metabolism of offspring.

中文翻译：

---

母体补充过氧化氢酶调节泌乳母猪及其后代脂肪酸代谢和抗氧化能力

简介和方法作为一种重要的抗氧化酶，过氧化氢酶（CAT）可以破坏细胞过氧化氢以减轻氧化应激。目前的研究旨在调查从妊娠晚期到哺乳期第 14 天母体补充 CAT 对母猪-仔猪轴抗氧化能力和脂肪酸代谢的影响。妊娠第95天，40头母猪分为对照组（CON）组（基础日粮喂养）和CAT组（基础日粮添加660 mg/kg CAT），饲喂实验于泌乳第14天结束。 结果CAT 组的泌乳母猪产奶量更高，抗氧化酶活性更高，包括 POD 和 GSH-Px（P< 0.05), 较低的血清 LDL 含量以及血浆 C18:3n3 含量 (P< 0.05）。此外，母体补充 CAT 改善了后代在哺乳期第 14 天的体重和平均增重（P< 0.05), 并通过降低相关酶活性 (如 T-AOC 和 CAT) 和改变基因表达水平来证明抗氧化能力。显着影响哺乳仔猪脂代谢，表现为升高血清ALT、CHOL和LDL（P< 0.05) 水平和调节血浆中长链脂肪酸 (MCFAs 和 LCFAs)，以及调节脂质代谢相关基因的表达。在哺乳期，进一步促进哺乳仔猪的生长。这些研究结果可能为母亲从妊娠晚期到哺乳期补充CAT以促进后代脂肪酸代谢提供参考价值。