An effective identification and discovery of fungal pigments is very important to illustrate the role of fungal pigments in the life process and conduce to the discovery of new bioactive and edible pigments. The phenotype combined with metabolomic and genomic (PMG) strategy led to the discovery and characterization of three new sorbicillinoid pigments, stasorbicillinoids A–C (1–3), and five known analogues (4–8) from the sponge-derived fungus Stagonospora sp. SYSU-MS7888. Their structures were elucidated by the application of spectroscopic methods (NMR, MS, UV, IR, and ECD) and modified Mosher’s method. Compounds 1 and 2 featured novel naphthone nuclei linked by two alkyl side chains possibly undergoing inter- and intramolecular Michael reactions. Compounds 1–8 exhibited potent anti-inflammatory activity with IC₅₀ values in the range of 3.56–22.8 μM. Furthermore, compound 2 inhibited the production of IL-1β, IL-6, and TNF-α in a dose-dependent manner. This study provides an effective strategy to accelerate the discovery of new fungal pigments and further exploration of their potential applications in different fields such as medicine and food industries.

中文翻译：

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从海绵衍生真菌 Stagonospora sp. 中靶向发现具有抗炎活性的山梨西林色素。SYSU-MS7888 使用 PMG 策略

真菌色素的有效鉴定和发现，对于阐明真菌色素在生命过程中的作用，有助于发现新的具有生物活性和可食用的色素具有十分重要的意义。表型与代谢组学和基因组学 (PMG) 策略相结合导致发现和表征了三种新的山梨西林素色素、司山梨西林素 A–C ( 1–3 ) 和来自海绵衍生真菌Stagonospora sp的五种已知类似物 ( 4–8 ) . SYSU-MS7888。通过应用光谱方法（NMR、MS、UV、IR 和 ECD）和改进的 Mosher 方法阐明了它们的结构。化合物1和2以可能发生分子间和分子内迈克尔反应的两个烷基侧链连接的新型萘酮核为特色。化合物1–8表现出有效的抗炎活性，IC ₅₀值在 3.56–22.8 μM 范围内。此外，化合物2以剂量依赖性方式抑制 IL-1β、IL-6 和 TNF-α 的产生。本研究为加速新型真菌色素的发现以及进一步探索其在医药、食品等不同领域的潜在应用提供了有效的策略。