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Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma
bioRxiv - Biochemistry Pub Date : 2022-11-20 , DOI: 10.1101/2022.11.20.517241
Brian Robbins, Jacob Perry, Mary Long, Rob E. Carpenter

Methamphetamine and its metabolite amphetamine are frequently abused drugs. Whether ob-tained legally or from clandestine laboratories it is of relevance to determine the chiral makeup of these drugs for investigative purpose. Although urine and oral fluid matrices are commonly of-fered, less available to independent laboratories are techniques to verify dextro (D-) or levo (L-) (meth)amphetamine from human K2EDTA plasma. This paper outlines the development and validation of a method that includes the addition of internal standard and a two-step liquid-liquid extraction to remove the analytes from human K2EDTA plasma by triple quadrupole mass spec-trometry (LC-MS/MS). The assay was validated according to the United States Food and Drug Administration and College of American Pathologists guidelines, including assessment of the following parameters in plasma validation samples: linear range, limit of detection, lower limit of quantitation, matrix effects, inter- and intra-day assay precision and accuracy, carry over, linearity of dilution, matrix effects and stability. The outcome is a validated and reliable method for the determination of D- and L- isomer concentration of meth(amphetamine) human plasma samples that can be easily adopted by independent clinical laboratories.

中文翻译:

人 K2EDTA 血浆中(甲基)苯丙胺的 D- 和 L- 异构体的分析

甲基苯丙胺及其代谢物苯丙胺是经常被滥用的药物。无论是合法获得还是从秘密实验室获得,确定这些药物的手性组成对于调查目的都是相关的。虽然通常提供尿液和口腔液基质,但独立实验室较少使用的技术是验证人 K2EDTA 血浆中的右旋 (D-) 或左旋 (L-)(甲基)苯丙胺。本文概述了一种方法的开发和验证,该方法包括添加内标和两步液-液萃取,通过三重四极质谱 (LC-MS/MS) 去除人 K2EDTA 血浆中的分析物。该测定根据美国食品和药物管理局和美国病理学家学会指南进行了验证,包括评估血浆验证样品中的以下参数:线性范围、检测限、定量下限、基质效应、日间和日内测定精度和准确度、结转、稀释线性、基质效应和稳定性。结果是一种经过验证且可靠的方法,用于测定甲基(苯丙胺)人血浆样品的 D- 和 L- 异构体浓度,独立临床实验室可以轻松采用。
更新日期:2022-11-22
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