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High precision, high throughput generation of droplets containing single cells
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-11-21 , DOI: 10.1039/d2lc00841f Jiande Zhou 1 , Amaury Wei 1 , Arnaud Bertsch 1 , Philippe Renaud 1
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-11-21 , DOI: 10.1039/d2lc00841f Jiande Zhou 1 , Amaury Wei 1 , Arnaud Bertsch 1 , Philippe Renaud 1
Affiliation
The Poisson limit is a major problem for the isolation of single cells in different single-cell technologies and applications. In droplet-based single-cell assays, a scheme that is increasingly popular, the intrinsic randomness during single-cell encapsulation in droplets requires most of the created droplets to be empty, which has a profound impact on the efficiency and throughput of such techniques, and on the predictability of the combinatory droplet assays. Here we present a simple passive microfluidic system overcoming this limitation with unprecedented efficacy, allowing the generation of single-cell droplets for a wide range of operating conditions, with extremely high throughput (more than 22 000 single-cell loaded droplets per minute) and with an extremely low fault ratio (doublets or empty droplets), applicable to any cells and deformable particles. This versatile technique will shift the paradigm of single-cell encapsulation and will impact single-cell sequencing, rare cell isolation, multicellular/bead studies in immunology or cancer biology, etc.
中文翻译:
高精度、高通量生成含有单细胞的液滴
泊松极限是不同单细胞技术和应用中单细胞分离的主要问题。在基于液滴的单细胞检测中,一种越来越流行的方案,液滴中单细胞封装过程中的内在随机性要求大部分创建的液滴是空的,这对此类技术的效率和通量具有深远的影响,以及组合液滴测定的可预测性。在这里,我们提出了一个简单的被动微流体系统,以前所未有的功效克服了这一限制,允许在广泛的操作条件下生成单细胞液滴,具有极高的吞吐量(每分钟超过 22000 个单细胞负载液滴)并且极低的故障率(双峰或空滴),适用于任何细胞和可变形粒子。这种多功能技术将改变单细胞封装的范式,并将影响单细胞测序、稀有细胞分离、免疫学或癌症生物学中的多细胞/微珠研究,等等
更新日期:2022-11-24
中文翻译:
高精度、高通量生成含有单细胞的液滴
泊松极限是不同单细胞技术和应用中单细胞分离的主要问题。在基于液滴的单细胞检测中,一种越来越流行的方案,液滴中单细胞封装过程中的内在随机性要求大部分创建的液滴是空的,这对此类技术的效率和通量具有深远的影响,以及组合液滴测定的可预测性。在这里,我们提出了一个简单的被动微流体系统,以前所未有的功效克服了这一限制,允许在广泛的操作条件下生成单细胞液滴,具有极高的吞吐量(每分钟超过 22000 个单细胞负载液滴)并且极低的故障率(双峰或空滴),适用于任何细胞和可变形粒子。这种多功能技术将改变单细胞封装的范式,并将影响单细胞测序、稀有细胞分离、免疫学或癌症生物学中的多细胞/微珠研究,等等