Localization of single fluorescent emitters is central for many physicochemical and biophysical measurements at the nanoscale and beyond ensemble averaging. Examples include single-molecule tracking and super-resolution imaging by single-molecule localization microscopy. Among the numerous localization methods available, MINFLUX constituted a breakthrough for achieving a ∼10-fold improvement in resolution over wide-field camera-based approaches, reaching the molecular scale at moderate photon counts. Since then, other related methods have been developed and a common framework has been established for single-molecule localization through sequential structured illumination. Now, fluorescence nanoscopy and tracking of single fluorophores with true nanometric resolution are sitting on solid conceptual and experimental ground. There is an ample scope of questions where they can be applied in the near future to gain new insight into the dynamics and photophysics of natural and synthetic systems at the nanometer scale.

中文翻译：

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通过顺序结构照明对单个荧光团进行纳米分辨率成像和跟踪

单个荧光发射器的定位对于纳米级和超越整体平均的许多物理化学和生物物理测量至关重要。示例包括通过单分子定位显微镜进行的单分子跟踪和超分辨率成像。在众多可用的定位方法中，MINFLUX 构成了一项突破，其分辨率比基于广角相机的方法提高了约 10 倍，在中等光子数下达到了分子尺度。从那时起，已经开发了其他相关方法，并建立了通​​过顺序结构照明进行单分子定位的通用框架。现在，荧光纳米显微镜和具有真正纳米分辨率的单个荧光团的跟踪在坚实的概念和实验基础上。