A simple and effective colorimetric detection of adenosine 5-triphosphate (ATP) in 100% aqueous media was developed based on an indicator displacement approach (IDA). A commercially available dye, Chlorophosphonazo III (CPA), was utilized as the indicator and the ATP detection was achieved using the complex of CPA with Mg²⁺ in a 2:1 stoichiometric ratio (CPA₂-Mg²⁺) through the regeneration of CPA by the binding of ATP to Mg²⁺. Upon addition of a series of anions to the CPA₂-Mg²⁺ complex, only the appearance of the solution of the complex with ATP exhibited a color change from blue to purple which can be detected by the naked eye. Moreover, the ATP recognition was not hampered by the presence of other anions. Hence, CPA₂-Mg²⁺ is efficient in ATP highly selective and sensitive colorimetric detection in 100% aqueous media.

基于指示剂置换法 (IDA)，开发了 100% 水性介质中 5-三磷酸腺苷 (ATP) 的简单有效的比色检测。使用市售染料偶氮氯膦 III (CPA) 作为指示剂，使用 CPA 与 Mg ²⁺的复合物以 2:1 的化学计量比 (CPA ₂ -Mg ²⁺ ) 通过再生实现 ATP 检测CPA 通过 ATP 与 Mg ²⁺的结合。_{在 CPA 2} -Mg ²⁺中加入一系列阴离子后络合物，只有与ATP的络合物溶液的外观表现出肉眼可以检测到的由蓝色到紫色的颜色变化。此外，ATP 识别不受其他阴离子的影响。因此，CPA ₂ -Mg ²⁺在100%水性介质中的ATP高选择性和灵敏比色检测中是有效的。