Recycling cellulase can reduce the cost of lignocellulosic enzymatic hydrolysis. Here, a lignin-grafted sulfobetaine (LSB) was first synthesized by grafting sulfobetaine (SB) on enzymatic hydrolysis lignin (EHL). LSB had a sensitive response of pH and temperature. LSB dissolved under the conditions of lignocellulosic enzymatic hydrolysis (pH 5.0, 50 °C). After hydrolysis, LSB co-precipitated with cellulase when lowering pH of the hydrolysate to 4.0 and cooling to 25 °C. When 3.0 g/L LSB-100 was added to the hydrolysis system of corncob residue (CCR), 70 % of amount of cellulase was saved. LSB had a remarkable response and stronger cellulase recovery capacity. This was attributed that carboxylate radical in LSB was protonated, and positive and negative ions of SB associated to form salt at 25 °C. This work provides a new idea for reducing the cost for preparing fermentable sugars from lignocellulose, and increasing the added value of EHL.

回收纤维素酶可以降低木质纤维素酶解的成本。在这里，首先通过在酶水解木质素 (EHL) 上接枝磺基甜菜碱 (SB) 合成了木质素接枝磺基甜菜碱 (LSB)。LSB对pH值和温度有敏感的反应。LSB 在木质纤维素酶水解 (pH 5.0, 50 °C) 的条件下溶解。水解后，当将水解产物的 pH 值降低至 4.0 并冷却至 25 °C 时，LSB 与纤维素酶共沉淀。当向玉米芯渣(CCR)水解体系中加入3.0 g/L LSB-100时，可节省70%的纤维素酶用量。LSB反应显着，纤维素酶回收能力更强。这归因于 LSB 中的羧酸根被质子化，SB 的正负离子在 25 °C 时结合形成盐。