Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2022-11-18 , DOI: 10.1016/j.aca.2022.340643 Wenjiao Zhou 1 , Lei Liao 1 , Xiaocheng Fan 1 , Jianglong Yao 1 , Bingying Jiang 1
Accurate and ultrasensitive detection of biomarkers is significance for the diagnosis of diseases at early stage. For this purpose, we herein develop a bidirectional dynamic DNA nano-device for amplified fluorescent detection of tumor marker of mucin 1 (MUC1). The nano-device is constructed by immobilizing two sets of DNA cascade catalytic probes on two opposite directions of a single-stranded DNA tracker to limit probe reactants to a compact space. Once target MUC1 binds to the aptamer sequence, the initiator DNA locked in the duplex DNA substrate can be released to induce DNA-initiated cascade hybridization reactions (DCHRs) simultaneously in two opposite directions along the tracker DNA, accompanying the displacement of two quencher labeled-DNA intermediate initiators to facilitate successively execution of DCHRs on the DNA nano-devices, which results in the separation of fluorophore (FAM) and quencher (Dabycl) to produce substantially recovered fluorescent signals for rapid and sensitive detection of MUC1 with a detection limit down to 0.18 pM. In addition, this strategy also exhibits high selectivity against other interfering proteins and potential application capacity in real serum samples, indicating its promising application prospects in disease diagnosis and treatment.
中文翻译:
可编程双向动态 DNA 纳米器件,用于准确和超灵敏地荧光检测血清中的痕量 MUC1 生物标志物
生物标志物的准确和超灵敏检测对于疾病的早期诊断具有重要意义。为此,我们在此开发了一种双向动态 DNA 纳米装置,用于放大荧光检测粘蛋白 1 (MUC1) 的肿瘤标记物。该纳米装置是通过在单链 DNA 跟踪器的两个相反方向上固定两组 DNA 级联催化探针来构建的,以将探针反应物限制在紧凑的空间内。一旦目标 MUC1 与适体序列结合,锁定在双链 DNA 底物中的起始 DNA 就会被释放,以沿着跟踪 DNA 在两个相反的方向同时诱导 DNA 起始的级联杂交反应 (DCHR),伴随着两个标记为-的猝灭剂的置换促进 DCHR 在 DNA 纳米器件上连续执行的 DNA 中间引发剂,这导致荧光团 (FAM) 和猝灭剂 (Dabycl) 分离,产生基本上恢复的荧光信号,用于快速、灵敏地检测 MUC1,检测限低至 0.18 pM。此外,该策略还表现出对其他干扰蛋白的高选择性和在真实血清样本中的潜在应用能力,表明其在疾病诊断和治疗中具有广阔的应用前景。