Ammonium perfluorooctanoate (APFOA) was used as a surfactant for the separation of free unsaturated C18 fatty acids by micellar electrokinetic chromatography. A simple background electrolyte of 50 mM APFOA water/methanol (90:10, v/v) at pH = 10 enabled the repeatable separation of oleic acid, elaidic acid, linoleic acid, and alpha-linolenic acid in less than 20 min. Separation conditions were optimized regarding various parameters (organic solvent, counterion, APFOA concentration, and pH). Because the repulsive interactions between fluorocarbon chains and hydrogenated chains are known to lead to segregation and phase separation, the choice of perfluorinated micelles to separate such perhydrogenated long-chain acids could appear astonishing. Therefore, the critical micelle concentration, the charge density, and the mobility of the micelles have been determined, resulting in a first description of the separation process.

中文翻译：

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全氟化胶束电动色谱分离不饱和C18脂肪酸：一、优化与分离工艺

全氟辛酸铵 (APFOA) 用作表面活性剂，用于通过胶束电动色谱法分离游离不饱和 C18 脂肪酸。pH = 10 的 50 mM APFOA 水/甲醇 (90:10, v/v) 的简单背景电解质能够在不到 20 分钟的时间内实现油酸、反油酸、亚油酸和 α-亚麻酸的可重复分离。分离条件针对各种参数（有机溶剂、抗衡离子、APFOA 浓度和 pH 值）进行了优化。由于已知碳氟化合物链和氢化链之间的排斥相互作用会导致分离和相分离，因此选择全氟化胶束来分离这种全氢化长链酸可能会令人吃惊。因此，临界胶束浓度、电荷密度、