Delivery systems smaller than 50 nm are advantageous for cancer prevention. In this study, curcumin was dissolved in shellac micelles following co-dissolving at pH 13.0 and neutralization using glucono-delta-lactone. With 5% w/v shellac and 0.5–5 mg/mL curcumin, the loading capacity and encapsulation efficiency were up to 8.0 and 92.6%, respectively, and the nanocapsules had an average diameter of 20 nm. Differential scanning calorimetry, FTIR spectroscopy, and fluorescence spectroscopy results confirmed the encapsulation of curcumin in an amorphous state in shellac micelles. The neutral nanocapsule dispersions maintained the particle dimension and had less than 10% curcumin degradation during 4 week storage at 4 °C. Nanoencapsulating curcumin enhanced in vitro bioavailability and antiproliferation activity against colon cancer cells. After simulated digestions, ∼60% of the nanoencapsulated curcumin was not available for intestinal absorption, nanocapsules retained their structure, and nanoencapsulated curcumin remained active against colon cancer cells, indicating the potential delivery for colorectal cancer prevention.

中文翻译：

---

载有姜黄素的虫胶胶束使用 pH 循环改善分散性、生物可及性和结肠递送的潜力

小于 50 nm 的递送系统有利于癌症预防。在这项研究中，姜黄素在 pH 13.0 下共溶解并使用葡糖酸-δ-内酯中和后溶解在虫胶胶束中。使用 5% w/v 虫胶和 0.5–5 mg/mL 姜黄素，载量和包封率分别高达 8.0% 和 92.6%，纳米胶囊的平均直径为 20 nm。差示扫描量热法、FTIR 光谱和荧光光谱结果证实姜黄素以无定形状态包封在虫胶胶束中。中性纳米胶囊分散体保持颗粒尺寸，在 4 °C 下储存 4 周期间姜黄素降解小于 10%。纳米胶囊化姜黄素增强体外生物利用度和抗结肠癌细胞增殖活性。